蛋白质结晶与相图

Protein crystallization and phase diagrams.

作者信息

Asherie Neer

机构信息

Department of Physics, Center for Materials Science and Engineering and Materials Processing Center, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA.

出版信息

Methods. 2004 Nov;34(3):266-72. doi: 10.1016/j.ymeth.2004.03.028.

DOI:10.1016/j.ymeth.2004.03.028

PMID:15325646

Abstract

The phase diagram is a map which represents the state of a material (e.g., solid and liquid) as a function of the ambient conditions (e.g., temperature and concentration). It is therefore a useful tool in processing many different classes of materials. In this article, methods to determine the phase diagram of an aqueous solution of a globular protein are described, focusing on the solid (crystal) and condensed liquid states. The use of the information contained in the phase diagram for protein crystallization is also discussed.

摘要

相图是一种图表，它表示材料的状态（例如，固体和液体）作为环境条件（例如，温度和浓度）的函数。因此，它是处理许多不同种类材料时的一种有用工具。在本文中，描述了确定球状蛋白质水溶液相图的方法，重点是固体（晶体）和凝聚液体状态。还讨论了将相图中包含的信息用于蛋白质结晶的情况。