Wei Dongwei, Wang Meng, Wang Hongdi, Liu Guijin, Fang Jun, Jiang Yanbin
School of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou 362000, China.
College of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Hangzhou Normal University, Hangzhou 311121, Zhejiang, China.
ACS Omega. 2022 Aug 22;7(35):31338-31347. doi: 10.1021/acsomega.2c03666. eCollection 2022 Sep 6.
Protein solubility is very important for protein crystallization, bioprocess development, and protein application. In this study, a method based on the stability of a protein dispersion system is proposed for fast assessment of protein solubility, which mainly involves ultrasonic dispersion, differential centrifugation, and spectral measurement (UDDCS) and curvature estimation. The appropriate ultrasonic time and centrifugal time were experimentally determined at first. The results show that the relationship between the standard deviation and the protein concentrations originally added accords with the modified exponential equation, and the corresponding concentration of the maximum curvature point is defined as the solubility of the protein. Lysozyme solubility data in NaCl aqueous solutions and zein solubility data in ethanol aqueous solutions are selected to verify the UDDCS method by comparing the data obtained by the UDDCS method and the results from references, and the results indicate that the UDDCS method is reliable, universal, and time-saving. Finally, measurements of zein solubility in NaOH solution and casein solubility in urea aqueous solution were conducted as test cases by the UDDCS method.
蛋白质溶解度对于蛋白质结晶、生物工艺开发和蛋白质应用非常重要。在本研究中，提出了一种基于蛋白质分散体系稳定性的方法，用于快速评估蛋白质溶解度，该方法主要包括超声分散、差速离心和光谱测量（UDDCS）以及曲率估计。首先通过实验确定了合适的超声时间和离心时间。结果表明，标准差与最初添加的蛋白质浓度之间的关系符合修正指数方程，并且将最大曲率点的相应浓度定义为蛋白质的溶解度。选择溶菌酶在NaCl水溶液中的溶解度数据和玉米醇溶蛋白在乙醇水溶液中的溶解度数据，通过比较UDDCS方法获得的数据与参考文献的结果来验证UDDCS方法，结果表明UDDCS方法可靠、通用且省时。最后，以UDDCS方法对玉米醇溶蛋白在NaOH溶液中的溶解度和酪蛋白在尿素水溶液中的溶解度进行了测试。
