大分子低温晶体学——在低温下冷却和安装蛋白质晶体的方法。
Macromolecular cryocrystallography--methods for cooling and mounting protein crystals at cryogenic temperatures.
作者信息
Pflugrath J W
机构信息
Rigaku/MSC, Inc., 9009 New Trails Drive, The Woodlands, TX 77381, USA.
出版信息
Methods. 2004 Nov;34(3):415-23. doi: 10.1016/j.ymeth.2004.03.032.
PMID:15325658
Abstract
Cryocrystallography is routinely used in macromolecular crystallography laboratories. The main advantage of X-ray diffraction data collection near 100K is that crystals display much less radiation damage than seen at room temperature. Techniques and tools are described to facilitate cryoprotecting and flash-cooling crystals for data collection.
摘要
低温晶体学在大分子晶体学实验室中经常使用。在接近100K的温度下收集X射线衍射数据的主要优点是，晶体显示出的辐射损伤比在室温下少得多。本文描述了有助于对晶体进行冷冻保护和快速冷却以进行数据收集的技术和工具。
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