Bearse Marcus A, Han Ying, Schneck Marilyn E, Barez Shirin, Jacobsen Carl, Adams Anthony J
School of Optometry and Vision Science Program, University of California, Berkeley, California 94720-2020, USA.
Invest Ophthalmol Vis Sci. 2004 Sep;45(9):3259-65. doi: 10.1167/iovs.04-0308.
To study retinal dysfunction in diabetes and early nonproliferative diabetic retinopathy (NPDR) using a new method to analyze local multifocal electroretinogram oscillatory potentials (mfOPs).
One eye of each of 26 normal subjects, 16 diabetic subjects without retinopathy (NoR), and 16 diabetic subjects with early NPDR was examined. Slow-flash multifocal electroretinograms (sf-mfERGs) were recorded from the central 45 degrees, and stereo fundus photographs of the diabetic eyes were taken. The first-order (K1), induced first-order (K1i), and second-order (K2) response components were extracted from each retinal location, and K1i and K2 were added to create Ks2. Responses from 35 contiguous areas were digitally filtered 90 to 225 Hz to isolate the mfOPs. The signal-to-noise ratio (SNR) of the mfOPs was calculated, and abnormality was defined as SNR below the fifth percentile of the normal subjects.
Combining the K1i and K2 components to form Ks2 before isolation of the mfOPs by digital filtering increased the SNR. Mean Ks2 and K1 mfOP SNRs were abnormal in 25% and 19% of the NoR eyes, respectively, and both were abnormal in 62% of the NPDR eyes. The retinal distributions of the local Ks2 and K1 mfOP abnormalities overlapped, but they differed. Furthermore, local Ks2 mfOP abnormalities were preferentially associated with retinal sites containing NPDR but K1 mfOP abnormalities were not.
The cells that contribute to the generation of local mfOPs are affected by diabetes and, to a greater degree, by early NPDR. The results suggest that fast adaptive mechanisms influencing the mfOPs are most abnormal at retinal sites containing NPDR.
采用一种新方法分析局部多焦视网膜电图振荡电位（mfOPs），以研究糖尿病及早期非增殖性糖尿病视网膜病变（NPDR）中的视网膜功能障碍。
对26名正常受试者、16名无视网膜病变的糖尿病受试者（NoR）以及16名患有早期NPDR的糖尿病受试者的单眼进行检查。从中央45度记录慢闪光多焦视网膜电图（sf-mfERGs），并拍摄糖尿病眼的立体眼底照片。从每个视网膜位置提取一阶（K1）、诱导一阶（K1i）和二阶（K2）反应成分，并将K1i和K2相加得到Ks2。对35个相邻区域的反应进行90至225Hz的数字滤波，以分离出mfOPs。计算mfOPs的信噪比（SNR），将SNR低于正常受试者第五百分位数定义为异常。
在通过数字滤波分离mfOPs之前，将K1i和K2成分组合形成Ks2可提高SNR。NoR组眼中，平均Ks2和K1 mfOP SNR分别有25%和19%异常，而NPDR组眼中两者均异常的比例为62%。局部Ks2和K1 mfOP异常的视网膜分布有重叠，但也存在差异。此外，局部Ks2 mfOP异常优先与含有NPDR的视网膜部位相关，而K1 mfOP异常则不然。
参与局部mfOPs产生的细胞受糖尿病影响，且在更大程度上受早期NPDR影响。结果表明，影响mfOPs的快速适应性机制在含有NPDR的视网膜部位异常最为明显。
