Kordyukova Larisa V, Ksenofontov Aleksander L, Serebryakova Marina V, Ovchinnikova Tatyana V, Fedorova Natalija V, Ivanova Valeria T, Baratova Ludmila A
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119992, Russia.
Protein Pept Lett. 2004 Aug;11(4):385-91. doi: 10.2174/0929866043406850.
MALDI-TOF MS and N-terminal amino acid sequencing allowed us to identify several fragments of the C-terminal peptide of Influenza A hemagglutinin (HA) containing transmembrane domains (TMD). These fragments were detected in the organic phase of chloroform-methanol extracts from bromelain-treated virus particles. Heterogeneous fatty acylation of the C-terminus was revealed. Tritium bombardment technique might open an opportunity for 3D structural investigation of the HA TMD in situ.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和N端氨基酸测序使我们能够鉴定出甲型流感血凝素（HA）含跨膜结构域（TMD）的C端肽的几个片段。这些片段在菠萝蛋白酶处理的病毒颗粒的氯仿-甲醇提取物的有机相中被检测到。揭示了C端的异质脂肪酰化。氚轰击技术可能为原位研究HA TMD的三维结构提供机会。
