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流感病毒血凝素的硬脂酰化：质谱分析揭示硬脂酸在跨膜半胱氨酸上的位点特异性附着。

S acylation of the hemagglutinin of influenza viruses: mass spectrometry reveals site-specific attachment of stearic acid to a transmembrane cysteine.

作者信息

Kordyukova Larisa V, Serebryakova Marina V, Baratova Ludmila A, Veit Michael

机构信息

A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119991, Russia.

出版信息

J Virol. 2008 Sep;82(18):9288-92. doi: 10.1128/JVI.00704-08. Epub 2008 Jul 2.

DOI:10.1128/JVI.00704-08
PMID:18596092
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2546910/
Abstract

S acylation of cysteines located in the transmembrane and/or cytoplasmic region of influenza virus hemagglutinins (HA) contributes to the membrane fusion and assembly of virions. Our results from using mass spectrometry (MS) show that influenza B virus HA possessing two cytoplasmic cysteines contains palmitate, whereas HA-esterase-fusion glycoprotein of influenza C virus having one transmembrane cysteine is stearoylated. HAs of influenza A virus having one transmembrane and two cytoplasmic cysteines contain both palmitate and stearate. MS analysis of recombinant viruses with deletions of individual cysteines, as well as tandem-MS sequencing, revealed the surprising result that stearate is exclusively attached to the cysteine positioned in the transmembrane region of HA.

摘要

流感病毒血凝素（HA）跨膜和/或细胞质区域中的半胱氨酸酰化作用有助于病毒粒子的膜融合和组装。我们使用质谱（MS）得到的结果表明，具有两个细胞质半胱氨酸的乙型流感病毒HA含有棕榈酸酯，而具有一个跨膜半胱氨酸的丙型流感病毒HA酯酶融合糖蛋白则被硬脂酰化。具有一个跨膜和两个细胞质半胱氨酸的甲型流感病毒HA同时含有棕榈酸酯和硬脂酸酯。对个别半胱氨酸缺失的重组病毒进行的MS分析以及串联MS测序揭示了一个惊人的结果，即硬脂酸酯仅附着于HA跨膜区域中的半胱氨酸上。

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本文引用的文献

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Nat Rev Mol Cell Biol. 2007 Jan;8(1):74-84. doi: 10.1038/nrm2084.
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Palmitoylation of influenza virus proteins.流感病毒蛋白的棕榈酰化作用。
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Mass spectrometric sequencing and acylation character analysis of C-terminal anchoring segment from Influenza A hemagglutinin.甲型流感血凝素C末端锚定片段的质谱测序及酰化特征分析
Eur J Mass Spectrom (Chichester). 2006;12(1):51-62. doi: 10.1255/ejms.792.
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Influenza virus hemagglutinin (H3 subtype) requires palmitoylation of its cytoplasmic tail for assembly: M1 proteins of two subtypes differ in their ability to support assembly.流感病毒血凝素（H3亚型）组装需要其胞质尾的棕榈酰化：两种亚型的M1蛋白在支持组装的能力上有所不同。
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Acylation-mediated membrane anchoring of avian influenza virus hemagglutinin is essential for fusion pore formation and virus infectivity.酰化介导的禽流感病毒血凝素膜锚定对于融合孔形成和病毒感染性至关重要。
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