Datta Priya, Thakur Archana, Mishra Bibhabati, Gupta Varsha
Department of Microbiology, Govind Ballah Pant Hospital, New Delhi, India.
Jpn J Infect Dis. 2004 Aug;57(4):146-9.
The incidence of infection due to extended spectrum beta-lactamases (ESBLs) producing Enterobacteriaceae has markedly increased in recent years. The traditional susceptibility methods lack sensitivity and/or specificity and this issue has prompted the search for an accurate test to detect the presence of ESBL. The present study included 300 bacterial strains and was undertaken to determine the prevalence of ESBL-producing strains. Here, compared three tests: a double disk synergy test (DDS), a three-dimensional test (3-D), and an inhibitor potentiated disk diffusion test (IPT); each test employed three different antibiotic discs, i.e., ceftazidime, ceftriaxone, and cefotaxime, in order to screen for ESBL strains. A strain was said to be an ESBL producer if it showed positive result(s) on any one of the three tests. The prevalence rate of ESBL in our hospital was 12.6% (38/300). IPT detected the most strains (34/38), followed by 3-D (23/38), and then DDS (15/38). The ceftriaxone disc was found to detect more ESBLs than either the ceftazidime or the cefotaxime disc.
近年来，产超广谱β-内酰胺酶（ESBLs）的肠杆菌科细菌引起的感染发生率显著增加。传统的药敏试验方法缺乏敏感性和/或特异性，这一问题促使人们寻找一种准确的检测方法来检测ESBL的存在。本研究纳入了300株细菌菌株，旨在确定产ESBL菌株的流行率。在此，比较了三种试验：双纸片协同试验（DDS）、三维试验（3-D）和抑制剂增强纸片扩散试验（IPT）；每种试验使用三种不同的抗生素纸片，即头孢他啶、头孢曲松和头孢噻肟，以筛选ESBL菌株。如果某菌株在三种试验中的任何一种试验中呈阳性结果，则该菌株被认为是产ESBL菌株。我院ESBL的流行率为12.6%（38/300）。IPT检测出的菌株最多（34/38），其次是3-D（23/38），然后是DDS（15/38）。发现头孢曲松纸片比头孢他啶或头孢噻肟纸片能检测出更多的ESBLs。
