Vallinoto Antonio Carlos R, Muto Nilton A, Pontes Gemilson S, Machado Luiz Fernando A, Azevedo Vânia N, dos Santos Sidney E B, Ribeiro-dos-Santos Andrea K C, Ishak Marluísa O G, Ishak Ricardo
Departamento de Patologia, Centro de Ciências Biologicas, Universidade Federal do Pará, Para, Brasil.
Jpn J Infect Dis. 2004 Aug;57(4):156-9.
Human T-lymphotropic virus type I (HTLV-I) infection was investigated in 168 Japanese immigrants (64 males and 104 females) living in the Tome-Acu county located in the State of Para, Brazil. The serological screening was performed using an enzyme-linked immunosorbent assay, and showed the presence of anti-HTLV in four women whose ages ranged from 50 to 88. Confirmation of infection and discrimination HTLV typing was performed using a nested PCR on the extracted DNA targeting the pX region. In three of the samples, infection was confirmed to be HTLV-I. Sequencing HTLV-I 5'LTR and the RFLP pattern using DraI and SacI endonucleases indicated that the virus is a member of the Cosmopolitan group. These three women originated from the Kyushu region, though two of the corresponding HTLV-I strains were phylogenetically related to the Japanese subgroup and the third to the Transcontinental subgroup, which probably reflects the geographical origin of the infected individuals. The Japanese community residing in the northern Brazil apparently have not contributed to increase the prevalence of HTLV-I in the country.
对居住在巴西帕拉州托梅-阿苏县的168名日本移民（64名男性和104名女性）进行了人类嗜T淋巴细胞病毒I型（HTLV-I）感染调查。采用酶联免疫吸附试验进行血清学筛查，结果显示4名年龄在50至88岁之间的女性体内存在抗HTLV。利用针对pX区域的巢式PCR对提取的DNA进行感染确认和HTLV分型鉴别。在其中3个样本中，感染被确认为HTLV-I。对HTLV-I 5'LTR进行测序以及使用DraI和SacI核酸内切酶的RFLP模式表明，该病毒是世界范围组的成员。这3名女性来自九州地区，尽管其中2株相应的HTLV-I毒株在系统发育上与日本亚组相关，第3株与跨大陆亚组相关，这可能反映了受感染个体的地理来源。居住在巴西北部的日本社区显然并未促使该国HTLV-I患病率上升。
