Senbon Shoichiro, Ota Atsushi, Tachibana Masao, Miyano Takashi
Graduate School of Science and Technology, Kobe University, Japan.
J Reprod Dev. 2004 Aug;50(4):439-44. doi: 10.1262/jrd.50.439.
Xenografting of ovarian tissue into immunodeficient mice has been used as a model to study the dynamics of follicular development and provides an alternative method for the production of mature oocytes. In a previous experiment, we demonstrated that xenografted bovine secondary follicles developed to the antral stage in severe combined immunodeficient (SCID) mice. In the present study, we examined the development of bovine secondary follicles (140-190 microm in diameter) grafted into ovariectomized mice in comparison with intact female mice as a control. At 4 weeks after grafting, several antral follicles ranging from 350 to 550 microm (457.6 +/- 50.8 microm) in diameter were found in the control mice, while a single large (larger than 2.5 mm) antral follicle and other small follicles were observed in every ovariectomized mouse. At 6 weeks after grafting, the mean diameter of morphologically normal follicles had further increased in the control group (591.8 +/- 132.0 microm). In ovariectomized mice, however, the mean diameter of follicles decreased (4 weeks: 864.2 +/- 988.2 microm; 6 weeks: 496.5 +/- 137.6 microm), since the single large antral follicle observed at 4 weeks had degenerated by 6 weeks. In control mice, more than 70% of follicles were morphologically normal and formed an antrum, and most of the follicles contained morphologically normal oocytes which grew to 122.5 +/- 2.2 microm. In ovariectomized mice, morphologically normal oocytes also grew larger than before grafting, but their survival rate was significantly lower than that in control mice. These results suggest that ovariectomy of host mice alters the developmental pattern of xenografted bovine secondary follicles to accelerate a single follicle to develop in the graft.
将卵巢组织异种移植到免疫缺陷小鼠体内已被用作研究卵泡发育动态的模型，并为成熟卵母细胞的产生提供了一种替代方法。在先前的实验中，我们证明了异种移植的牛次级卵泡在严重联合免疫缺陷（SCID）小鼠中发育到窦状卵泡阶段。在本研究中，我们将直径为140 - 190微米的牛次级卵泡移植到去卵巢小鼠体内，并与完整雌性小鼠作为对照进行比较，观察其发育情况。移植后4周，对照小鼠中发现了几个直径为350至550微米（457.6±50.8微米）的窦状卵泡，而在每只去卵巢小鼠中观察到一个大的（大于2.5毫米）窦状卵泡和其他小卵泡。移植后6周，对照组中形态正常卵泡的平均直径进一步增加（591.8±132.0微米）。然而，在去卵巢小鼠中，卵泡的平均直径减小了（4周：864.2±988.2微米；6周：496.5±137.6微米），因为在4周时观察到的单个大窦状卵泡到6周时已经退化。在对照小鼠中，超过70%的卵泡形态正常并形成了卵泡腔，并且大多数卵泡含有形态正常的卵母细胞，其生长到122.5±2.2微米。在去卵巢小鼠中，形态正常的卵母细胞也比移植前长得更大，但其存活率显著低于对照小鼠。这些结果表明，宿主小鼠的去卵巢改变了异种移植的牛次级卵泡的发育模式，加速了移植中单个卵泡的发育。
