Gook D A, McCully B A, Edgar D H, McBain J C
Reproductive Biology Unit, Royal Women's Hospital, Australia.
Hum Reprod. 2001 Mar;16(3):417-22. doi: 10.1093/humrep/16.3.417.
This study reports the first gross morphological and histological evidence of antral follicle development in human ovarian tissue following cryopreservation. Human ovarian tissue was cryopreserved using propanediol and sucrose and grafted under the renal capsule of bilaterally oophorectomized severe combined immunodeficient (SCID) mice. Follicles at all stages of development were observed in the grafted tissue whereas, prior to grafting, only primary and primordial follicles were present. Antral follicles were rarely observed on grafts examined <20 weeks after grafting either non-frozen tissue (fresh, 1/7) or cryopreserved tissue (0/11). In contrast, development of at least one antral follicle was evident on the majority of sites > or = 20 weeks after grafting (fresh 7/9, cryopreserved 18/24). Antral follicle diameters ranged from 0.1 to 5.0 mm. Histological examination of these antral follicles indicated normal follicular morphology, i.e. antral cavities encapsulated by concentric layers of theca and granulosa cells. Pedicles containing germinal vesicle oocytes were observed protruding from the granulosa cell layers. The development and morphology of the cryopreserved and fresh tissue following grafting was similar.
本研究报告了冷冻保存后人卵巢组织中窦卵泡发育的首个大体形态学和组织学证据。使用丙二醇和蔗糖对人卵巢组织进行冷冻保存，并移植到双侧卵巢切除的重度联合免疫缺陷（SCID）小鼠的肾包膜下。在移植组织中观察到了各个发育阶段的卵泡，而在移植前，仅存在初级卵泡和原始卵泡。在移植后<20周检查的移植物中，无论是未冷冻组织（新鲜组织，1/7）还是冷冻保存组织（0/11），很少观察到窦卵泡。相比之下，在移植后≥20周的大多数部位，至少有一个窦卵泡发育明显（新鲜组织7/9，冷冻保存组织18/24）。窦卵泡直径范围为0.1至5.0毫米。对这些窦卵泡的组织学检查表明卵泡形态正常，即窦腔被卵泡膜细胞和颗粒细胞的同心层包裹。观察到含有生发泡卵母细胞的蒂从颗粒细胞层突出。移植后冷冻保存组织和新鲜组织的发育和形态相似。
