Hosoe Misa, Furusawa Tadashi, Noguchi Junko, Tokunaga Tomoyuki
Reproductive Biology Research Unit, Division of Animal Sciences, National Institute of Agrobiological Sciences, Ibaraki, Japan.
Reprod Med Biol. 2008 Feb 1;7(1):45-54. doi: 10.1111/j.1447-0578.2007.00200.x. eCollection 2008 Mar.
Various researchers have studied xenografting ovarian tissues into immunodeficient mice to accelerate the follicular growth of several mammalian species. In this study, the authors focused on the following three points in growing follicles in transplanted ovarian tissues under kidney capsules: the effects of the storage conditions of the donor ovarian tissues, the effects of donor age on the survival rates of grafted mouse ovaries, and the methods used to grow the follicles of xenografted bovine ovaries. When ovaries stored for 0, 6, 12 or 24 h at 4°C and at room temperature were transplanted under the kidney capsules of immunodeficient mice, fewer mouse and rabbit grafts survived following 24 h storage. The survival rates of bovine grafts were relatively low for all storage times. When mouse ovaries were held for 24 h at 4°C or at room temperature, low-temperature storage effectively improved the survival rates of the grafts. Although the survival rates of grafted genital ridges containing premeiotic germ cells from fetuses and grafted ovaries from mice 0, 10, 20, 40 and 80 days after birth were similar among donors of different ages, the cleavage rate of oocytes following insemination was significantly lower in the grafts from the ovaries of 80-day-old mice. Antral follicles formed in surviving bovine ovarian grafts. Cumulus-oocyte complexes were collected from the grafted ovaries of fetuses and calves, and the oocytes reached the metaphase II stage following culture, but they did not develop to the pronuclear stage after fertilization. Our findings provide basic data on xenografting ovarian tissues into immunodeficient mice to accelerate the growth of follicles. (Reprod Med Biol 2008; : 45-54).
多位研究人员研究了将卵巢组织异种移植到免疫缺陷小鼠体内，以加速几种哺乳动物物种卵泡生长的情况。在本研究中，作者关注了肾被膜下移植卵巢组织中卵泡生长的以下三个要点：供体卵巢组织的保存条件的影响、供体年龄对移植小鼠卵巢存活率的影响，以及异种移植牛卵巢卵泡生长所采用的方法。当在4°C和室温下分别保存0、6、12或24小时的卵巢移植到免疫缺陷小鼠的肾被膜下时，保存24小时后存活的小鼠和兔移植物较少。在所有保存时间下，牛移植物的存活率都相对较低。当小鼠卵巢在4°C或室温下保存24小时时，低温保存有效地提高了移植物的存活率。尽管来自胎儿的含有减数分裂前生殖细胞的移植生殖嵴和出生后0、10、20、40和80天小鼠的移植卵巢在不同年龄供体中的存活率相似，但80日龄小鼠卵巢移植物中受精后卵母细胞的分裂率显著较低。存活的牛卵巢移植物中形成了窦卵泡。从胎儿和小牛的移植卵巢中收集卵丘 - 卵母细胞复合体，培养后卵母细胞达到减数分裂中期II期，但受精后它们没有发育到原核期。我们的研究结果为将卵巢组织异种移植到免疫缺陷小鼠体内以加速卵泡生长提供了基础数据。（《生殖医学与生物学》2008年；：45 - 54）
