Hynek Radovan, Kuckova Stepanka, Hradilova Janka, Kodicek Milan
Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech Republic.
Rapid Commun Mass Spectrom. 2004;18(17):1896-900. doi: 10.1002/rcm.1570.
Identification of materials in color layers of paintings is necessary for correct decisions concerning restoration procedures as well as proving the authenticity of the painting. The proteins are usually important components of the painting layers. In this paper it has been demonstrated that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) can be used for fast and reliable identification of proteins in color layers even in old, highly aged matrices. The digestion can be easily performed directly on silica wafers which are routinely used for infrared analysis. The amount of material necessary for such an analysis is extremely small. Peptide mass mapping using digestion with trypsin followed by MALDI-TOFMS and identification of the protein was successfully used for determination of the binder from a painting of the 19th century.
识别绘画色彩层中的材料对于做出有关修复程序的正确决策以及证明绘画的真伪至关重要。蛋白质通常是绘画层的重要组成部分。本文已证明，基质辅助激光解吸/电离飞行时间质谱（MALDI-TOFMS）可用于快速、可靠地识别色彩层中的蛋白质，即使是在陈旧、年代久远的基质中。消化过程可直接在常用于红外分析的硅片上轻松进行。这种分析所需的材料量极少。使用胰蛋白酶消化后进行MALDI-TOFMS的肽质量图谱分析和蛋白质鉴定已成功用于确定一幅19世纪绘画的粘合剂。
