Peng Song, Ahlmann Norman, Kunze Kerstin, Nigge Walter, Edler Michael, Hoffmann Thorsten, Franzke Joachim
Institute for Analytical Sciences, Bunsen-Kirchhoff-Strasse 11, 44139 Dortmund, Germany.
Rapid Commun Mass Spectrom. 2004;18(16):1803-8. doi: 10.1002/rcm.1556.
The desorption of an analyte by a continuous wave diode laser from a porous surface of a thin-layer plate covered with a graphite suspension is presented. The thermally desorbed analyte molecules are ionized in the gas phase by a corona discharge at atmospheric pressure. Therefore, both essential processes--the desorption and the ionization of analyte molecules, which are often performed in one step--are separated. The target preparation is easy and fast since no additional extraction process is required. The mass spectrometric background signal was mostly limited to the low mass range showing no interference with typical compounds of interest. In this study, the calmative and antihypertensive drug reserpine was chosen as model analyte, which is often used for specification of mass spectrometers. No fragmentation was observed because of efficient collisional cooling under atmospheric pressure. The influence of diode laser power and the composition of the graphite suspension were investigated, and a primary optimization was performed.
本文介绍了利用连续波二极管激光器从覆盖有石墨悬浮液的薄层板多孔表面解吸分析物的方法。热解吸的分析物分子在大气压下通过电晕放电在气相中被电离。因此，通常在一步中完成的两个基本过程——分析物分子的解吸和电离——被分开了。由于不需要额外的萃取过程，靶标制备简单快速。质谱背景信号大多局限于低质量范围，对典型的目标化合物没有干扰。在本研究中，选择镇静和降压药物利血平作为模型分析物，它常用于质谱仪的性能指标测定。由于在大气压下有高效的碰撞冷却，未观察到碎片化现象。研究了二极管激光功率和石墨悬浮液组成的影响，并进行了初步优化。
