Slepchuk N A
Thermoregulation and Bioenergetics Group, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, 6 Makarov Bank, 199034 St. Petersburg, Russia.
Neurosci Behav Physiol. 2004 Jun;34(5):453-7. doi: 10.1023/b:neab.0000022629.79624.4c.
White rats were cooled in water until respiratory arrest, which appeared at a brain temperature of 17.62 +/- 0.16 degrees C; animals were then removed from the water to a room at a temperature of 19-20 degrees C. Experimental animals were given 1 ml of 0.6% EDTA, which binds calcium ions, 1 min after the onset of respiratory arrest; controls received 1 ml of physiological saline. Electrical heaters were used to warm the medulla oblongata; respiratory recovery occurred at 6.0 +/- 0.3 min from the onset of respiratory arrest, when the medulla oblongata temperature was 18.62 +/- 0.15 degrees C. In controls, heating of the medulla oblongata to 18.84 +/- 0.17 degrees C did not restore respiration. EDTA bound calcium ions, facilitating transport of the excess quantities of these ions from the cell cytosol to the intercellular medium. Decreases in the cytosol calcium ion concentration to normal restored the normal regulation of metabolism in cells.
将白色大鼠置于水中冷却直至呼吸停止，呼吸停止出现在脑温为17.62±0.16摄氏度时；然后将动物从水中取出，置于温度为19 - 20摄氏度的房间。在呼吸停止开始1分钟后，给实验动物注射1毫升0.6%的乙二胺四乙酸（EDTA），其可结合钙离子；对照组注射1毫升生理盐水。使用电加热器加热延髓；呼吸停止开始后6.0±0.3分钟，当延髓温度为18.62±0.15摄氏度时，呼吸恢复。在对照组中，将延髓加热至18.84±0.17摄氏度并未恢复呼吸。EDTA结合钙离子，促进这些过量离子从细胞胞质溶胶转运至细胞间介质。胞质溶胶钙离子浓度降至正常水平可恢复细胞内代谢的正常调节。
