Wojciech Ł, Staroniewicz Z, Jakubczak A, Ugorski M
Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Agriculture University Norwid St. 31, 50-375 Wroclaw, Poland.
J Vet Med B Infect Dis Vet Public Health. 2004 Jun;51(5):238-44. doi: 10.1111/j.1439-0450.2004.00762.x.
Thirty-five Yersinia enterocolitica strains isolated from humans, pigs and foxes were analysed by genotyping including intergenic transcribed sequence (ITS) profiling, REP- and ERIC-PCR. ERIC-PCR revealed the presence of seven different genotypes. Amplification of the 16S-23S rDNA spacer region by ITS profiling gave similar results with nine different genotypes. REP-PCR was found to be more discriminatory for typing of Y. enterocolitica than ERIC-PCR and ITS profiling. Fifteen different DNA patterns were obtained by this technique. Based on data obtained by three methods it was found that: (i) Y. enterocolitica strains belonging to the same serotype can represent different genotypes and vice versa; (ii) isolates recovered from humans, pigs and foxes exhibit limited heterogeneity and, independent of the origin, one or two prevailing genotypes were always observed; and (iii) many human Y. enterocolitica isolates shared common genotypes with porcine isolates.
对从人、猪和狐狸中分离出的35株小肠结肠炎耶尔森菌菌株进行了基因分型分析，包括基因间转录序列（ITS）分析、REP-PCR和ERIC-PCR。ERIC-PCR显示存在7种不同的基因型。通过ITS分析对16S-23S rDNA间隔区进行扩增，得到了9种不同基因型的类似结果。发现REP-PCR对小肠结肠炎耶尔森菌分型的鉴别能力比ERIC-PCR和ITS分析更强。通过该技术获得了15种不同的DNA图谱。根据三种方法获得的数据发现：（i）属于同一血清型的小肠结肠炎耶尔森菌菌株可能代表不同的基因型，反之亦然；（ii）从人、猪和狐狸中分离出的菌株表现出有限的异质性，且无论来源如何，总是观察到一两种主要基因型；（iii）许多人类小肠结肠炎耶尔森菌分离株与猪分离株具有共同的基因型。
