Paixão Renata, Moreno Luisa Zanolli, Sena de Gobbi Débora Dirani, Raimundo Daniele Cristine, Ferreira Thais Sebastiana Porfida, Spindola Maria Garcia, Hofer Ernesto, Falavina Dos Reis Cristhiane Moura, Matté Maria Helena, Micke Moreno Andrea
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Butantã, 05508-270 São Paulo, SP, Brazil ; Faculdade de Medicina Veterinária, Faculdades Metropolitanas Unidas (FMU), Rua Ministro Nelson Hungria 541, 05690-050 São Paulo, SP, Brazil.
J Pathog. 2013;2013:521510. doi: 10.1155/2013/521510. Epub 2013 May 30.
Yersinia enterocolitica is a foodborne pathogen that causes illness in humans and animals. The biotype 4/O:3 has been commonly associated with yersiniosis and is characterized by the presence of chromosomal and extra-chromosomal virulence genes. Molecular typing methods have been successfully used to characterize Y. enterocolitica genetic heterogeneity and to study the epidemiology of the bacteria from different origins. In this study, 320 Y. enterocolitica biotype 4/O:3 isolates originating in pigs and slaughterhouses were characterized according to the virulence profile, and 61 isolates were typified through SE-AFLP, ERIC-PCR, and PFGE techniques. The majority of the isolates originated from pigs, and the predominant virulence profile was ail+ virF+ rfbC+ ystA+, representing 83.4% of the tested isolates. All of the Y. enterocolitica 4/O:3 isolates were positive for at least ystA gene. The SE-AFLP and ERIC-PCR patterns were highly homogeneous. The SE-AFLP was more discriminative than the ERIC-PCR and tended to cluster isolates according to the slaughterhouse. Despite the limited genetic diversity of Y. enterocolitica 4/O:3, PFGE was shown to be the most discriminative technique considering one band of difference. Fattening pigs proved to be an important reservoir of Y. enterocolitica biotype 4/O:3 carrying virulence genes.
小肠结肠炎耶尔森菌是一种食源性病原体，可导致人和动物患病。生物型4/O:3通常与耶尔森菌病相关，其特征是存在染色体和染色体外毒力基因。分子分型方法已成功用于表征小肠结肠炎耶尔森菌的遗传异质性，并研究来自不同来源的该细菌的流行病学。在本研究中，根据毒力谱对320株源自猪和屠宰场的小肠结肠炎耶尔森菌生物型4/O:3分离株进行了表征，并通过SE-AFLP、ERIC-PCR和PFGE技术对61株分离株进行了分型。大多数分离株源自猪，主要毒力谱为ail+ virF+ rfbC+ ystA+，占测试分离株的83.4%。所有小肠结肠炎耶尔森菌4/O:3分离株至少ystA基因呈阳性。SE-AFLP和ERIC-PCR图谱高度一致。SE-AFLP比ERIC-PCR更具鉴别力，并且倾向于根据屠宰场对分离株进行聚类。尽管小肠结肠炎耶尔森菌4/O:3的遗传多样性有限，但考虑到一条带的差异，PFGE被证明是最具鉴别力的技术。育肥猪被证明是携带毒力基因的小肠结肠炎耶尔森菌生物型4/O:3的重要储存宿主。
