用于功能性肾脏成像以诊断急性肾衰竭的基于多胺树枝状大分子的磁共振成像造影剂。
Polyamine dendrimer-based MRI contrast agents for functional kidney imaging to diagnose acute renal failure.
作者信息
Kobayashi Hisataka, Jo Sang-Kyung, Kawamoto Satomi, Yasuda Hideo, Hu Xuzhen, Knopp Michael V, Brechbiel Martin W, Choyke Peter L, Star Robert A
机构信息
Metabolism Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
出版信息
J Magn Reson Imaging. 2004 Sep;20(3):512-8. doi: 10.1002/jmri.20147.
PURPOSE
To choose an efficacious renal functional MRI contrast agent to image early renal tubular damage. We synthesized and compared smaller polyamine dendrimer-based MRI contrast agents (<60 kD) that, unlike Gd-[DTPA], transiently accumulate in renal tubules and can be used to visualize renal structural and functional damage.
MATERIALS AND METHODS
Six dendrimer-based MRI contrast agents smaller than 60 kD were studied by high resolution dynamic micro-MRI and compared to Gd-[DTPA]-dimeglumine and Gadomer-17. The best agent, DAB-G2, was further tested in a mouse ischemia/reperfusion model to validate its efficacy.
RESULTS
Despite unequal renal clearance rates, all polyamine dendrimer agents visualized the renal functional anatomy of the mice better than Gd-[DTPA]-dimeglumine and Gadomer-17. DAB-G2 was excreted most rapidly, yet was able to visualize mild renal tubular injury very early after injury.
CONCLUSION
DAB-G2 was found to be the best candidate for functional kidney imaging and enabled early diagnosis of acute renal injury.
目的
选择一种有效的肾功能磁共振成像（MRI）造影剂，用于早期肾小管损伤成像。我们合成并比较了基于较小聚胺树枝状大分子的MRI造影剂（<60 kDa），与钆喷酸葡胺（Gd-[DTPA]）不同，这些造影剂可在肾小管中短暂蓄积，可用于可视化肾脏结构和功能损伤。
材料与方法
通过高分辨率动态显微MRI研究了6种小于60 kDa的基于树枝状大分子的MRI造影剂，并与钆喷酸葡胺二甲基葡胺和Gadomer-17进行比较。最佳造影剂DAB-G2在小鼠缺血/再灌注模型中进一步测试，以验证其有效性。
结果
尽管肾脏清除率不同，但所有基于聚胺树枝状大分子的造影剂在显示小鼠肾功能解剖结构方面均优于钆喷酸葡胺二甲基葡胺和Gadomer-17。DAB-G2排泄最快，但能够在损伤后很早阶段就显示出轻度肾小管损伤。
结论
发现DAB-G2是肾功能成像的最佳候选者，能够实现急性肾损伤的早期诊断。
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