Jin Jiyang, Teng Gaojun, Feng Yi, Wu Yanping, Jin Qindi, Wang Yu, Wang Zhen, Lu Qin, Jiang Yibo, Wang Shengqi, Chen Feng, Marchal Guy, Ni Yicheng
Department of Radiology, Zhongda Hospital of Southeast University, Nanjing, China.
Cardiovasc Intervent Radiol. 2007 Mar-Apr;30(2):248-56. doi: 10.1007/s00270-006-0004-0.
To compare a necrosis-avid contrast agent (NACA) bis-Gd-DTPA-pamoic acid derivative (ECIII-60) after intracoronary delivery with an extracellular agent Gd-DTPA after intravenous injection on magnetic resonance imaging (MRI) in a swine model of acute reperfused myocardial infarction (MI).
Eight pigs underwent 90 min of transcatheter coronary balloon occlusion and 60 min of reperfusion. After intravenous injection of Gd-DTPA at a dose of 0.2 mmol/kg, all pigs were scanned with T1-weighted MRI until the delayed enhancement of MI disappeared. Then they were intracoronarily infused with ECIII-60 at 0.0025 mmol/kg and imaged for 5 hr. Signal intensity, infarct-over-normal contrast ratio and relative infarct size were quantified, compared, and correlated with the results of postmortem MRI and triphenyltetrazolium chloride (TTC) histochemical staining.
A contrast ratio over 3.0 was induced by both Gd-DTPA and ECIII-60. However, while the delayed enhancement with Gd-DTPA virtually vanished in 1 hr, ECIII-60 at an 80x smaller dose depicted the MI accurately over 5 hr as proven by ex vivo MRI and TTC staining.
Both Gd-DTPA and ECIII-60 strongly enhanced acute MI. Comparing with fading contrast in a narrow time window with intravenous Gd-DTPA, intracoronary ECIII-60 persistently demarcated the acute MI, indicating a potential method for postprocedural assessment of myocardial viability after coronary interventions.
在猪急性再灌注心肌梗死（MI）模型中，通过磁共振成像（MRI）比较冠状动脉内注射坏死亲和性造影剂（NACA）双钆 - 二乙三胺五乙酸 - 帕莫酸衍生物（ECIII - 60）与静脉注射细胞外造影剂钆 - 二乙三胺五乙酸后的情况。
八头猪接受90分钟的经导管冠状动脉球囊闭塞和60分钟的再灌注。以0.2 mmol/kg的剂量静脉注射钆 - 二乙三胺五乙酸后，所有猪均用T1加权MRI扫描，直至MI的延迟强化消失。然后以0.0025 mmol/kg的剂量冠状动脉内注入ECIII - 60，并成像5小时。对信号强度、梗死与正常组织的对比率和相对梗死面积进行量化、比较，并与死后MRI和氯化三苯基四氮唑（TTC）组织化学染色结果相关联。
钆 - 二乙三胺五乙酸和ECIII - 60均诱导出超过3.0的对比率。然而，虽然钆 - 二乙三胺五乙酸的延迟强化在1小时内几乎消失，但剂量小80倍的ECIII - 60在5小时内准确描绘了MI，体外MRI和TTC染色证明了这一点。
钆 - 二乙三胺五乙酸和ECIII - 60均强烈增强急性MI。与静脉注射钆 - 二乙三胺五乙酸在狭窄时间窗口内逐渐消退的对比不同，冠状动脉内注射的ECIII - 60持续界定急性MI，表明这是一种冠状动脉介入术后评估心肌活力的潜在方法。
