Songping Liang
College of Life Sciences, Hunan Normal University, Changsha, China.
Methods Mol Biol. 2010;615:75-85. doi: 10.1007/978-1-60761-535-4_6.
Spider venom contains a complex mixture of components with a large range of molecular masses (0.1-60 kDa) exhibiting a diverse array of actions. Most of these components are proteinaceous molecules - biologically active proteins and peptides. Proteomics profiling of spider venoms (the components with MW >10 kDa) could be achieved through conventional 2-DE-based proteomics methods combined with MS or MS/MS detection. Peptidomic profiling (of the components with MW below ~10 kDa) is usually achieved through off-line separation by a combination of ion-exchange and reverse-phase chromatography, and it relies more heavily on de novo sequencing by Edman degradation or MS/MS for peptide identification.
蜘蛛毒液含有多种分子量范围很大（0.1 - 60 kDa）的成分的复杂混合物，这些成分具有各种各样的作用。这些成分大多是蛋白质类分子——生物活性蛋白和肽。蜘蛛毒液的蛋白质组分析（分子量>10 kDa的成分）可通过基于传统二维电泳的蛋白质组学方法结合质谱或串联质谱检测来实现。肽组分析（分子量低于约10 kDa的成分）通常通过离子交换和反相色谱联用的离线分离来完成，并且在肽鉴定方面更依赖于埃德曼降解或串联质谱的从头测序。
