Vannas A, Sweeney D F, Holden B A, Sapyska E, Salonen E M, Vaheri A
Cornea and Contact Lens Research Unit, School of Optometry, University of New South Wales, Sydney, Australia.
Curr Eye Res. 1992 Mar;11(3):243-51. doi: 10.3109/02713689209001775.
Plasmin, a proteolytic enzyme, has been detected in the tears of patients experiencing anterior ocular disease, and during contact lens wear. Using a radial caseinolysis procedure, we examined tear plasmin levels in 66 patients who were wearing soft and rigid lenses for daily and extended wear. Compared to non-contact lens wearers, patients wearing soft and rigid lenses for extended wear were significantly more likely to exhibit tear plasmin activity. Eight hours of open-eye thick HEMA lens wear did not induce tear plasmin activity in a group of 10 subjects. However, significant increases in tear plasmin activity were recorded after short-term (1 hour) eye closure with and without lens wear, and following overnight (8 hours) eye closure without lens wear. Overnight eye closure also resulted in significantly increased numbers of epithelial cells and leucocytes in the tear fluid. Our results suggest that increased tear plasmin activity during extended contact lens wear may be attributable to the effects of eye closure rather than hypoxia or the presence of the contact lens per se.
纤溶酶是一种蛋白水解酶，已在患有眼前部疾病的患者眼泪中以及佩戴隐形眼镜期间检测到。我们采用径向酪蛋白溶解法，对66名日常佩戴和长期佩戴软性及硬性隐形眼镜的患者的泪液纤溶酶水平进行了检测。与不佩戴隐形眼镜者相比，长期佩戴软性和硬性隐形眼镜的患者泪液纤溶酶活性明显更高。在一组10名受试者中，睁眼佩戴厚HEMA镜片8小时并未诱导泪液纤溶酶活性。然而，在短期（1小时）闭眼（无论是否佩戴镜片）以及过夜（8小时）闭眼且不佩戴镜片后，泪液纤溶酶活性均显著增加。过夜闭眼还导致泪液中上皮细胞和白细胞数量显著增加。我们的研究结果表明，长期佩戴隐形眼镜期间泪液纤溶酶活性增加可能归因于闭眼的影响，而非缺氧或隐形眼镜本身的存在。
