Stapleton F, Willcox M D, Morris C A, Sweeney D F
Cornea and Contact Lens Research Unit, School of Optometry, University of New South Wales, Sydney, Australia.
Curr Eye Res. 1998 Feb;17(2):183-8. doi: 10.1076/ceyr.17.2.183.5597.
Tear protein composition alters during eye closure, by becoming rich in secretory IgA (sIgA) and certain complement proteins. This may reflect altered ocular defense mechanisms during eye closure. Since overnight wear of contact lenses (CLs) is associated with an increased risk of corneal infection and inflammation, this study aimed to quantify tear protein changes with overnight soft CL wear.
Non-stimulated tears were collected from 9 CL wearers prior to CL wear (baseline), after daily CL wear, and after 8 h sleep. Lenses were removed following wear and were extracted in 80% urea at 95 degrees C. Secretory IgA, complement C3 and C4, were measured using ELISA and total protein using the Pierce BCA assay. Assays were performed on tear samples and CL extracts.
Baseline tear protein concentrations were: 0 total tear protein (9.37 +/- 2.97 mg/mL), C3 (4.4 +/- 2.1 micrograms/mL), C4 (0.1 +/- 0.1 micrograms/mL), and sIgA (0.84 +/- 0.34 mg/mL). There were no differences in any protein levels between daily CL wear and no CL wear (p > 0.05). Following sleep, protein concentrations were: total tear protein (43.64 +/- 24.30 mg/mL), C3 (72.5 +/- 49.9 micrograms/mL), C4 (6.7 +/- 5.2 micrograms/mL), and sIgA (5.53 +/- 5.15 mg/mL). Total protein extracted from CLs after daily wear was 90 +/- 27 micrograms/CL and, after overnight wear, 152 +/- 24 micrograms/CL. Negligible levels of C3, C4 and sIgA were recovered from CL extracts.
Uncomplicated daily use of soft CLs does not appear to alter certain tear proteins compared with baseline levels. Uptake of these proteins by the CL does not appear to deplete the tear protein levels. Overnight levels of all tear proteins were increased, compared to daily CL wear.
在闭眼过程中，泪液蛋白质成分会发生变化，变得富含分泌型免疫球蛋白A（sIgA）和某些补体蛋白。这可能反映了闭眼期间眼部防御机制的改变。由于过夜佩戴隐形眼镜（CL）与角膜感染和炎症风险增加有关，本研究旨在量化过夜佩戴软性CL后泪液蛋白质的变化。
从9名CL佩戴者在佩戴CL前（基线）、每日佩戴CL后以及睡眠8小时后收集非刺激性泪液。佩戴后取出镜片，并在95摄氏度下用80%尿素进行提取。使用酶联免疫吸附测定法（ELISA）测量分泌型IgA、补体C3和C4，并使用皮尔斯BCA测定法测量总蛋白。对泪液样本和CL提取物进行检测。
基线泪液蛋白质浓度为：总泪液蛋白（9.37±2.97毫克/毫升）、C3（4.4±2.1微克/毫升）、C4（0.1±0.1微克/毫升）和sIgA（0.84±0.34毫克/毫升）。每日佩戴CL与不佩戴CL之间的任何蛋白质水平均无差异（p>0.05）。睡眠后，蛋白质浓度为：总泪液蛋白（43.64±24.30毫克/毫升）、C3（72.5±49.9微克/毫升）、C4（6.7±5.2微克/毫升）和sIgA（5.53±5.15毫克/毫升）。每日佩戴后从CL中提取的总蛋白为90±27微克/CL，过夜佩戴后为152±24微克/CL。从CL提取物中回收的C3、C4和sIgA水平可忽略不计。
与基线水平相比，单纯每日使用软性CL似乎不会改变某些泪液蛋白质。CL对这些蛋白质的摄取似乎不会耗尽泪液蛋白质水平。与每日佩戴CL相比，过夜后所有泪液蛋白质水平均升高。
