Shin W S, Kang M W, Kang J H, Choi M K, Ahn B M, Kim J K, Sun H S, Min K W
Division of Infectious Diseases, Catholic University Medical College, Seoul, Korea.
Am J Clin Pathol. 1996 Feb;105(2):174-81. doi: 10.1093/ajcp/105.2.174.
Epstein-Barr virus (EBV)-associated gastric carcinomas have been reported from various regions of the world. Epstein-Barr virus appears to be pathogenetically related to some gastric carcinomas. To determine the incidence of EBV association with gastric carcinomas among Koreans, the authors have studied EBV genome expression in 89 consecutive patients with gastric carcinomas diagnosed at the Catholic University Hospitals in Seoul, Korea, using in situ hybridization (ISH) for EBV-encoded small RNAs (EBERs), and immunohistochemistry for EBV latent membrane proteins (LMP) and CD21 antigen on paraffin sections. Thirty-seven gastric specimens with benign ulcer disease were used controls. EBV-encoded small RNAs were expressed in tumor cell nuclei in 12 patients (13.5%). None of the controls or benign portions of the cases were positive. In the positive cases, all tumor cell nuclei were uniformly stained and the staining intensity was strong. Immunohistochemistry for LMP was positive in 3 of 12 EBERs positive patients and none of EBERs negative patients. EBV latent membrane proteins was localized only in the lymphoid cells infiltrating the tumor in two patients, and tumor cells as well as infiltrating lymphoid cells in one patient. These results indicate that the rate of EBV association with gastric carcinomas in Koreans is relatively high and comparable to other Far Eastern Asian regions. The expression pattern in EBV-associated gastric carcinomas is similar to those of nasopharyngeal carcinomas in which clonality analysis using specific probes to the tandem repeat region of EBV yielded single episomal bands suggesting that EBV infection in EBV-associated gastric carcinomas are also clonal and pathogenetically related to the neoplasm. However, the mechanism of tumorigenesis remains to be elucidated.
世界各地均有关于爱泼斯坦-巴尔病毒（EBV）相关胃癌的报道。爱泼斯坦-巴尔病毒似乎在某些胃癌的发病机制中发挥作用。为确定韩国人群中EBV相关性胃癌的发病率，作者对韩国首尔天主教大学医院连续诊断的89例胃癌患者进行了研究，采用原位杂交（ISH）检测EBV编码的小RNA（EBERs），并对石蜡切片上的EBV潜伏膜蛋白（LMP）和CD21抗原进行免疫组化检测。选取37例良性溃疡病胃标本作为对照。12例患者（13.5%）的肿瘤细胞核中检测到EBV编码的小RNA表达。对照标本或病例的良性部分均为阴性。在阳性病例中，所有肿瘤细胞核均被均匀染色且染色强度较强。12例EBERs阳性患者中有3例LMP免疫组化呈阳性，而EBERs阴性患者均为阴性。EBV潜伏膜蛋白仅在2例患者肿瘤内浸润的淋巴细胞中定位，在1例患者的肿瘤细胞及浸润淋巴细胞中均有定位。这些结果表明，韩国人群中EBV相关性胃癌的发生率相对较高，与其他东亚地区相当。EBV相关胃癌的表达模式与鼻咽癌相似，对EBV串联重复区域使用特异性探针进行克隆性分析时，EBV相关胃癌产生单一条带，提示EBV相关胃癌中的EBV感染也是克隆性的，且在发病机制上与肿瘤相关。然而，其肿瘤发生机制仍有待阐明。
