Liang Hao, Liang Yu-zhen, Chen Hui, Yu Zhi-qing, Su Jie-han, Wu Zhi-bin, Qin Jin-yao
School of Public Health, Guangxi Medical University, Nanning 530021, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Dec;17(4):351-3.
To further study the role of human cytomegalovirus (HCMV) infection in the pathogenesis of the type 2 diabetes mellitus.
HCMV DNA levels in sera from 29 type 2 diabetic patients and 23 controls were measured by quantitative polymerase chain reaction (PCR), and comparative analyses was made between HCMV DNA and T cell subsets, blood glucose (BG), insulin (Ins) and C peptide (C-P).
The levels of HCMV DNA were (1.81+/-1.67) x 10(8) copies/ml for type 2 diabetic patients, a level significantly higher than that (5.50+/-4.30) x 10(7) copies/ml of controls. The percentage of CD8 for type 2 diabetic patients with positive HCMV DNA was 29.53%+/-2.00%, being much higher than that for controls (27.13%+/-4.12%), while the ratio of CD4/CD8 1.24+/-0.05 was significantly lower than that 1.41+/-0.10 of controls. Fasting C-P of type 2 diabetic patients with positive HCMV DNA was far lower than that of those with negative HCMV DNA, but the differences of BG and Ins between the two groups were not significant.
Active HCMV infection of type 2 diabetic patients may suppress cellular immunity and its influence on the pathogenesis of the type 2 diabetes mellitus should be further studied.
进一步研究人巨细胞病毒（HCMV）感染在2型糖尿病发病机制中的作用。
采用定量聚合酶链反应（PCR）检测29例2型糖尿病患者和23例对照者血清中的HCMV DNA水平，并对HCMV DNA与T细胞亚群、血糖（BG）、胰岛素（Ins）和C肽（C-P）进行比较分析。
2型糖尿病患者的HCMV DNA水平为（1.81±1.67）×10⁸拷贝/ml，显著高于对照组的（5.50±4.30）×10⁷拷贝/ml。HCMV DNA阳性的2型糖尿病患者CD8百分比为29.53%±2.00%，远高于对照组的（27.13%±4.12%），而CD4/CD8比值1.24±0.05显著低于对照组的1.41±0.10。HCMV DNA阳性的2型糖尿病患者空腹C-P远低于HCMV DNA阴性者，但两组间BG和Ins的差异无统计学意义。
2型糖尿病患者的HCMV活动性感染可能抑制细胞免疫，其对2型糖尿病发病机制的影响有待进一步研究。
