Zheng X-Q, Zhu F, Shi W-W, Lin A, Yan W-H
Department of Laboratory Medicine, The Second Hospital, Wenzhou Medical College, Zhejiang, China.
Tissue Antigens. 2009 Oct;74(4):317-21. doi: 10.1111/j.1399-0039.2009.01312.x. Epub 2009 Jul 17.
Human leukocyte antigen-G (HLA-G) expression is a potential factor for the pathogenesis of virus infection. A 14 bp insertion/deletion polymorphism (rs16375) in the 3'-untranslated region of the HLA-G gene is involved in the stability of HLA-G mRNA and HLA-G protein expression. Therefore, the HLA-G 14 bp polymorphism might be involved in human cytomegalovirus (hCMV) infection. To test a possible association between the HLA-G 14 bp deletion/insertion polymorphism and the active hCMV infection, in this study, a total of 54 patients with active hCMV infection and 165 age- and sex-matched, unrelated, normal Chinese Han population were genotyped for the 14 bp insertion/deletion polymorphism. Association of 14 bp polymorphism with hCMV urine DNA copies and the odds ratio (OR) of the polymorphism as a risk factor for active hCMV infection were analyzed. Our results showed that the prevalence of -14 bp/ -14 bp genotype in active hCMV patients was markedly increased [P(c) = 0.00034, OR = 3.31, 95% confidence interval (CI): 1.77-6.18], and similar significance was also observed for the frequency of -14 bp allele (P c = 0.0023, OR = 2.24, 95% CI: 1.38-3.64) when compared with that of healthy controls. Furthermore, urine hCMV DNA copies in patients with the -14 bp/ -14 bp genotype were significantly higher than those in patients with the +14 bp/ +14 bp genotype (P = 0.041). Our findings support a potential role of HLA-G 14 bp insertion/deletion polymorphism as a susceptible factor for the active hCMV infection.
人类白细胞抗原-G（HLA-G）表达是病毒感染发病机制的一个潜在因素。HLA-G基因3'-非翻译区的一个14bp插入/缺失多态性（rs16375）与HLA-G mRNA的稳定性及HLA-G蛋白表达有关。因此，HLA-G 14bp多态性可能与人类巨细胞病毒（hCMV）感染有关。为检测HLA-G 14bp缺失/插入多态性与活动性hCMV感染之间可能存在的关联，在本研究中，对54例活动性hCMV感染患者以及165名年龄和性别匹配、无亲缘关系的正常中国汉族人群进行了14bp插入/缺失多态性的基因分型。分析了14bp多态性与hCMV尿液DNA拷贝数的关联以及该多态性作为活动性hCMV感染危险因素的比值比（OR）。我们的结果显示，活动性hCMV患者中-14bp / -14bp基因型的患病率显著增加[P(c)=0.00034，OR = 3.31，95%置信区间（CI）：1.77 - 6.18]，与健康对照相比，-14bp等位基因频率也观察到类似的显著性（P c = 0.0023，OR = 2.24，95% CI：1.38 - 3.64）。此外，-14bp / -14bp基因型患者的尿液hCMV DNA拷贝数显著高于+14bp / +14bp基因型患者（P = 0.041）。我们的研究结果支持HLA-G 14bp插入/缺失多态性作为活动性hCMV感染易感因素的潜在作用。
