Wu Kun-He, Zhou Qing-Kui, Huang Jian-Hong, Lai Ri-Quan, Lin Fei-Hong, Li Bing, Zhang Chang-Bins, Zhou Wei-Ning, Zhu Zhao-Ping
Department of Pathology, Maternal and Child Health Hospital of Guangdong Province, Guangzhou, Guangdong 510010, China.
Zhonghua Nan Ke Xue. 2007 Dec;13(12):1075-9.
To study the infection of human cytomegalovirus (HCMV) and herpes simplex virus type II (HSV-I) and the morphological characteristics of the infected spermatogenic cells in the semen of infertile men.
We washed and concentrated the spermatogenic cells obtained from 83 semen samples of infertile men, extracted DNA and then screened HCMV and HSV-II by polymerase chain reaction (PCR). Immunocytochemistry (ICC) was used to detect the expression of correlative virus antigens of the positive semen cells, and the cytology smear was employed to observe the morphological changes of the spermatogenic cells under the microscope after cytology staining.
Of all the semen samples, 8 were HCMV positive, 4 HSV-II positive, but none were both HCMV and HSV-II positive. HCMV late antigens were positively and HCMV early antigens negatively expressed in the spermatogenic cells of the 8 HCMV positive cases. In the 4 HSV-II positive cases, 3 were positively and 1 weakly positively expressed. In the semen of the 12 positive cases were found large numbers of immature spermatogenic cells, with different manifestations of apoptosis, such as chromatin pycnosis, vacuoles, damaged nuclear membrane, and apoptotic bodies, but without virus infection-induced specific morphological alteration. Sperm concentration of the positive group was significantly lower than that of the negative (P < 0. 05).
Spermatogenic cells infected by HCMV and HSV-II may cause pathologic lesions and affect spermatogenesis. Morphologically, the infected spermatogenic cells may undergo some pathologic alteration, such as apoptosis. The rate of HCMV infection is higher among infertile males with pathologic cells in the semen.
研究不育男性精液中人类巨细胞病毒（HCMV）和单纯疱疹病毒II型（HSV-II）感染情况以及感染后生精细胞的形态学特征。
对83例不育男性精液标本获取的生精细胞进行洗涤和浓缩，提取DNA，然后采用聚合酶链反应（PCR）筛查HCMV和HSV-II。运用免疫细胞化学（ICC）检测阳性精液细胞相关病毒抗原的表达，采用细胞学涂片在细胞学染色后于显微镜下观察生精细胞的形态变化。
所有精液标本中，8例HCMV阳性，4例HSV-II阳性，但无HCMV和HSV-II均阳性者。8例HCMV阳性病例的生精细胞中HCMV晚期抗原呈阳性表达，HCMV早期抗原呈阴性表达。4例HSV-II阳性病例中，3例呈阳性表达，1例弱阳性表达。12例阳性病例的精液中发现大量未成熟生精细胞，有不同表现的凋亡，如染色质固缩、空泡形成、核膜受损及凋亡小体，但无病毒感染诱导的特异性形态改变。阳性组精子浓度显著低于阴性组（P<0.05）。
HCMV和HSV-II感染生精细胞可能导致病理损害并影响精子发生。形态学上，感染的生精细胞可能发生一些病理改变，如凋亡。精液中有病理细胞的不育男性中HCMV感染率较高。
