Verica Joseph A, Maximova Siela N, Strem Mary D, Carlson John E, Bailey Bryan A, Guiltinan Mark J
Department of Horticulture, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
Plant Cell Rep. 2004 Nov;23(6):404-13. doi: 10.1007/s00299-004-0852-5. Epub 2004 Aug 31.
Pathogenic diseases represent a major constraint to the growth and yield of cacao (Theobroma cacao L.). Ongoing research on model plant systems has revealed that defense responses are activated via signaling pathways mediated by endogenous signaling molecules such as salicylic acid, jasmonic acid and ethylene. Activation of plant defenses is associated with changes in the expression of large numbers of genes. To gain a better understanding of defense responses in cacao, we have employed suppressive subtractive hybridization (SSH) cDNA libraries, macroarray hybridization analysis, high throughput DNA sequencing and bioinformatics to identify cacao genes induced by these signaling molecules. Additionally, we investigated gene activation by a phytotoxic elicitor-like protein, Nep1. We have identified a unigene set of 1,256 members, including 330 members representing genes induced during the defense response.
致病病害是可可树（Theobroma cacao L.）生长和产量的主要限制因素。对模式植物系统的持续研究表明，防御反应是通过由水杨酸、茉莉酸和乙烯等内源性信号分子介导的信号通路激活的。植物防御的激活与大量基因表达的变化有关。为了更好地了解可可树的防御反应，我们采用了抑制性消减杂交（SSH）cDNA文库、宏阵列杂交分析、高通量DNA测序和生物信息学来鉴定由这些信号分子诱导的可可树基因。此外，我们研究了一种类植物毒素激发子蛋白Nep1对基因的激活作用。我们已经鉴定出一个由1256个成员组成的单基因集，其中包括330个代表防御反应期间诱导基因的成员。
