Lima L S, Gramacho K P, Carels N, Novais R, Gaiotto F A, Lopes U V, Gesteira A S, Zaidan H A, Cascardo J C M, Pires J L, Micheli F
Centro de Pesquisas do Cacau, Itabuna, BA, Brasil.
Genet Mol Res. 2009 Jul 14;8(3):799-808. doi: 10.4238/vol8-3gmr603.
In order to increase the efficiency of cacao tree resistance to witches' broom disease, which is caused by Moniliophthora perniciosa (Tricholomataceae), we looked for molecular markers that could help in the selection of resistant cacao genotypes. Among the different markers useful for developing marker-assisted selection, single nucleotide polymorphisms (SNPs) constitute the most common type of sequence difference between alleles and can be easily detected by in silico analysis from expressed sequence tag libraries. We report the first detection and analysis of SNPs from cacao-M. perniciosa interaction expressed sequence tags, using bioinformatics. Selection based on analysis of these SNPs should be useful for developing cacao varieties resistant to this devastating disease.
为了提高可可树对由可可毛色二孢（多孔菌科）引起的丛枝病的抗性效率，我们寻找了有助于选择抗性可可基因型的分子标记。在用于开发标记辅助选择的不同标记中，单核苷酸多态性（SNP）构成了等位基因之间最常见的序列差异类型，并且可以通过从表达序列标签文库进行电子分析轻松检测到。我们使用生物信息学报告了首次从可可 - 可可毛色二孢相互作用表达序列标签中检测和分析SNP。基于这些SNP分析的选择对于培育抗这种毁灭性疾病的可可品种应该是有用的。
