Peek Simon E, Hartmann Faye A, Thomas Chet B, Nordlund Kenneth V
Departments of Medical Sciences, School of Veterinary Medicine, University of Wisconsin. Madison, WI 53706, USA.
J Am Vet Med Assoc. 2004 Aug 15;225(4):574-7. doi: 10.2460/javma.2004.225.574.
To determine whether Salmonella spp could be isolated from the environment of free stall dairies in Wisconsin without any history of clinical salmonellosis and determine the serotype and antimicrobial susceptibility of any Salmonella isolates recovered from the environment.
Cross-sectional study.
20 free stall dairies with no history of clinical salmonellosis.
Dairy owners completed a questionnaire regarding management and production practices. Multiple swab samples were obtained from throughout the free stall facilities and submitted for bacterial culture for Salmonella spp. Odds ratios were calculated to compare herd-level risk factors between dairies from which Salmonella organisms were isolated and herds from which Salmonella organisms were not isolated.
Salmonella organisms were isolated from 9 of the 20 (45%) dairies. Salmonella serotype Meleagridis was isolated from 4 dairies, S. Meleagridis and S. Kentucky were isolated from 2 dairies, S. Meleagridis and S. Cyprus were isolated from 1 dairy, S. Cerro was isolated from 1 dairy, and S. Corvallis was isolated from 1 dairy. All isolates were susceptible to all antimicrobial agents tested. None of the potential risk factors analyzed demonstrated a significant association with an increased likelihood of isolating Salmonella spp.
Environmental Salmonella contamination was demonstrated on free stall dairies with no history of clinical salmonellosis.
确定在威斯康星州无临床沙门氏菌病病史的散栏式奶牛场环境中是否能分离出沙门氏菌属，并确定从环境中分离出的任何沙门氏菌分离株的血清型和抗菌药物敏感性。
横断面研究。
20个无临床沙门氏菌病病史的散栏式奶牛场。
奶牛场主完成了一份关于管理和生产实践的问卷。从整个散栏设施中采集多个拭子样本，并提交进行沙门氏菌属细菌培养。计算比值比，以比较分离出沙门氏菌的奶牛场和未分离出沙门氏菌的奶牛场之间畜群水平的风险因素。
20个奶牛场中有9个（45%）分离出了沙门氏菌。从4个奶牛场分离出了火鸡沙门氏菌血清型，从2个奶牛场分离出了火鸡沙门氏菌和肯塔基沙门氏菌，从1个奶牛场分离出了火鸡沙门氏菌和塞浦路斯沙门氏菌，从1个奶牛场分离出了塞罗沙门氏菌，从1个奶牛场分离出了科瓦利斯沙门氏菌。所有分离株对所有测试的抗菌药物均敏感。分析的所有潜在风险因素均未显示与分离出沙门氏菌属的可能性增加有显著关联。
在无临床沙门氏菌病病史的散栏式奶牛场中证实了环境沙门氏菌污染。
