Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
Foodborne Pathog Dis. 2010 Jun;7(6):659-65. doi: 10.1089/fpd.2009.0462.
The focus of this study was Salmonella enterica serotype Cerro, a potentially emerging pathogen of cattle. Our objectives were to document the within-herd prevalence of Salmonella Cerro among a sample of New York dairy herds, to describe the antimicrobial resistance patterns and pulsed-field gel electrophoresis types of the isolates, and to elucidate the status of this serotype as a bovine pathogen. Data were collected prospectively from dairy herds throughout New York that had at least 150 lactating cows and that received clinical service from participating veterinarians. Following enrollment, Salmonella surveillance consisted of both environmental screening and disease monitoring within the herd. Herds positive by either environmental or fecal culture were sampled during three visits to estimate the within-herd prevalence of Salmonella. Among 57 enrolled herds, 44 (77%) yielded Salmonella-positive samples during the study period. Of these, 20 herds (46%) were positive for Salmonella Cerro. Upon follow-up sampling for estimation of prevalence, Cerro was identified in 10 of the 20 herds; the median within-herd Cerro prevalence was 17%, with a maximum of 53%. Antimicrobial resistance ranged from zero to nine drugs, and eight (40%) of the Cerro-positive farms generated drug-resistant isolates. Eight XbaI pulsed-field gel electrophoresis types were represented among 116 isolates tested, although 89% of these isolates shared the predominant type. Among herds with clinical cases, cattle that had signs consistent with salmonellosis were more likely to test positive for Cerro than apparently healthy cattle, as estimated by a logistic regression model that controlled for herd as a random effect (odds ratio: 3.9). There is little in the literature concerning Salmonella Cerro, and published reports suggest an absence of disease association in cattle. However, in our region there has been an apparent increase in the prevalence of this serotype among cattle with salmonellosis. Other Salmonella serotypes important to bovine health have emerged to become leading causes of human foodborne disease, and close monitoring of Cerro is warranted.
本研究的重点是肠沙门氏菌血清型塞拉，一种潜在的牛传染病病原体。我们的目标是记录纽约奶牛场样本中沙门氏菌塞拉的群内流行率，描述分离株的抗生素耐药模式和脉冲场凝胶电泳类型，并阐明该血清型作为牛病原体的状态。数据是从纽约各地的奶牛场收集的，这些奶牛场至少有 150 头泌乳牛，并由参与的兽医提供临床服务。登记后，沙门氏菌监测包括群内的环境筛查和疾病监测。通过环境或粪便培养呈阳性的牛群在三次访问期间采样，以估计群内沙门氏菌的流行率。在 57 个登记的牛群中，有 44 个（77%）在研究期间采集到沙门氏菌阳性样本。其中，20 个牛群（46%）为沙门氏菌塞拉阳性。在随后的采样以估计流行率时，在 20 个牛群中的 10 个牛群中鉴定出了塞拉。群内塞拉的中位数流行率为 17%，最高为 53%。抗生素耐药范围从无到 9 种药物，8 个（40%）塞拉阳性农场产生了耐药分离株。在测试的 116 个分离株中，有 8 个 XbaI 脉冲场凝胶电泳类型，尽管其中 89%的分离株具有主要类型。在有临床病例的牛群中，与沙门氏菌病一致的症状的牛比明显健康的牛更有可能对塞拉呈阳性，这是通过控制牛群作为随机效应的逻辑回归模型估计的（优势比：3.9）。关于塞拉氏菌的文献很少，已发表的报告表明该菌与牛的疾病无关。然而，在我们的地区，沙门氏菌塞拉在患有沙门氏菌病的牛中的流行率似乎有所增加。其他对牛健康很重要的沙门氏菌血清型已成为人类食源性疾病的主要原因，因此有必要对塞拉氏菌进行密切监测。
