人类胚胎干细胞的内皮细胞和造血细胞命运起源于具有成血管细胞特性的原始内皮。
Endothelial and hematopoietic cell fate of human embryonic stem cells originates from primitive endothelium with hemangioblastic properties.
作者信息
Wang Lisheng, Li Li, Shojaei Farbod, Levac Krysta, Cerdan Chantal, Menendez Pablo, Martin Tanya, Rouleau Anne, Bhatia Mickie
机构信息
Robarts Research Institute, Krembil Centre for Stem Cell Biology and Regenerative Medicine, 100 Perth Drive, London, Ontario N6A 5K8, Canada.
出版信息
Immunity. 2004 Jul;21(1):31-41. doi: 10.1016/j.immuni.2004.06.006.
The cellular organization and relationships among precursors that initiate embryonic angiogenesis and hematopoiesis in the human have yet to be characterized. Here, we identify a subpopulation of primitive endothelial-like cells derived from human embryonic stem cells (hESCs) that express PECAM-1, Flk-1, and VE-cadherin, but not CD45 (CD45negPFV cells), and that are uniquely responsible for endothelial and hematopoietic development. Molecular profiling of CD45negPFV cells is consistent with endothelial and hematopoietic competency. Clonal isolation demonstrates that the CD45negPFV population includes bipotent cells with endothelial and hematopoietic capacity. We suggest that human hematopoiesis and endothelial maturation originate exclusively from a subset of embryonic endothelium that possesses hemangioblastic properties and offers a model system to study these lineage relationships in the human.
人类胚胎血管生成和造血起始过程中前体细胞的细胞组织及相互关系尚未得到明确描述。在此，我们鉴定出了一类源自人胚胎干细胞（hESCs）的原始内皮样细胞亚群，这些细胞表达血小板内皮细胞黏附分子-1（PECAM-1）、血管内皮生长因子受体1（Flk-1）和血管内皮钙黏蛋白（VE-cadherin），但不表达CD45（CD45阴性PFV细胞），且它们对内皮和造血发育具有独特的作用。CD45阴性PFV细胞的分子谱分析与内皮和造血能力一致。克隆分离表明，CD45阴性PFV群体包含具有内皮和造血能力的双能细胞。我们认为，人类造血和内皮成熟完全起源于具有成血管细胞特性的胚胎内皮亚群，这为研究人类这些谱系关系提供了一个模型系统。
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