Jandik Petr, Cheng Jun, Avdalovic Nebojsa
Dionex Corporation, 445 Lakeside Drive, Sunnyvale, CA 94085, USA.
J Biochem Biophys Methods. 2004 Sep 30;60(3):191-203. doi: 10.1016/j.jbbm.2004.01.003.
A review is presented of recent developments in the area of analysis of amino acid-carbohydrate mixtures. Based on its broad selectivity, the AminoPac PA10 column exhibits a remarkable capability to perform simultaneous separations of amino acids and carbohydrates. This ability is further enhanced by the equal sensitivity for carbohydrates and amino acids exhibited by the "amino acid" integrated pulsed electrochemical detection (IPAD) waveforms. Equimolar levels of carbohydrates and amino acids are separated either by optimized elution gradients alone or by a combination of modified gradients and a bi-modal IPAD waveform. Samples containing large amounts of carbohydrates may be analyzed after suitable sample preparation. Both a manual off-line method and a fully automatic on-line method are discussed. In addition, we will review the application of these methods to various types of samples, including cell culture media, glycoprotein hydrolysates, beverages, condiments and soil extracts.
