用于分离阪崎肠杆菌的荧光选择性鉴别培养基。
Fluorogenic selective and differential medium for isolation of Enterobacter sakazakii.
作者信息
Oh Se-Wook, Kang Dong-Hyun
机构信息
Department of Food Science and Human Nutrition, Washington State University, Pullman, WA 99164-6376, USA.
出版信息
Appl Environ Microbiol. 2004 Sep;70(9):5692-4. doi: 10.1128/AEM.70.9.5692-5694.2004.
Abstract
4-Methylumbelliferyl-alpha-D-glucoside, the fluorogenic substrate of alpha-glucosidase, was used as a selective marker to develop a differential medium for Enterobacter sakazakii. This bacterium showed strong fluorogenic characteristics clearly distinguishable from other microorganisms. On the basis of reducing background noise, an optimum basal medium and nitrogen source were selected. Incubation conditions were optimized.
摘要
4-甲基伞形酮基-α-D-葡萄糖苷，α-葡萄糖苷酶的荧光底物，被用作一种选择性标记物来开发用于阪崎肠杆菌的鉴别培养基。这种细菌表现出明显区别于其他微生物的强荧光特性。在降低背景噪音的基础上，选择了最佳基础培养基和氮源。对培养条件进行了优化。
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