Dombkowski Ryan A, Russell Michael J, Schulman Alexis A, Doellman Meredith M, Olson Kenneth R
South Bend Center for Medical Education, Indiana University School of Medicine, University of Notre Dame, Notre Dame, IN 46556, USA.
Am J Physiol Regul Integr Comp Physiol. 2005 Jan;288(1):R243-52. doi: 10.1152/ajpregu.00324.2004. Epub 2004 Sep 2.
Hydrogen sulfide (H(2)S) is a recently identified endogenous vasodilator in mammals. In steelhead/rainbow trout (Oncorhynchus mykiss, Osteichthyes), H(2)S produces both dose-dependent dilation and a unique dose-dependent constriction. In this study, we examined H(2)S vasoactivity in all vertebrate classes to determine whether H(2)S is universally vasoactive and to identify phylogenetic and/or environmental trends. H(2)S was generated from NaHS and examined in unstimulated and precontracted systemic and, when applicable, pulmonary arteries (PA) from Pacific hagfish (Eptatretus stouti, Agnatha), sea lamprey (Petromyzon marinus, Agnatha), sandbar shark (Carcharhinus milberti, Chondrichthyes), marine toad (Bufo marinus, Amphibia), American alligator (Alligator mississippiensis, Reptilia), Pekin duck (Anas platyrhynchos domesticus, Aves), and white rat (Rattus rattus, Mammalia). In otherwise unstimulated vessels, NaHS produced 1) a dose-dependent relaxation in Pacific hagfish dorsal aorta; 2) a dose-dependent contraction in sea lamprey dorsal aorta, marine toad aorta, alligator aorta and PA, duck aorta, and rat thoracic aorta; 3) a threshold relaxation in shark ventral aorta, dorsal aorta, and afferent branchial artery; and 4) a multiphasic contraction-relaxation-contraction in the marine toad PA, duck PA, and rat PA. Precontraction of these vessels with another agonist did not affect the general pattern of NaHS vasoactivity with the exception of the rat aorta, where relaxation was now dominant. These results show that H(2)S is a phylogenetically ancient and versatile vasoregulatory molecule that appears to have been opportunistically engaged to suit both organ-specific and species-specific homeostatic requirements.
硫化氢（H₂S）是最近在哺乳动物中发现的一种内源性血管舒张剂。在虹鳟鱼（Oncorhynchus mykiss，硬骨鱼纲）中，H₂S既能产生剂量依赖性舒张，也能产生独特的剂量依赖性收缩。在本研究中，我们检测了所有脊椎动物类群中H₂S的血管活性，以确定H₂S是否普遍具有血管活性，并识别系统发育和/或环境趋势。H₂S由硫氢化钠产生，并在未受刺激和预先收缩的体循环以及（如适用）来自太平洋盲鳗（Eptatretus stouti，无颌纲）、海七鳃鳗（Petromyzon marinus，无颌纲）、沙虎鲨（Carcharhinus milberti，软骨鱼纲）、海蟾蜍（Bufo marinus，两栖纲）、美国短吻鳄（Alligator mississippiensis，爬行纲）、北京鸭（Anas platyrhynchos domesticus，鸟纲）和白鼠（Rattus rattus，哺乳纲）的肺动脉（PA）中进行检测。在其他未受刺激的血管中，硫氢化钠产生了以下效应：1）在太平洋盲鳗背主动脉中产生剂量依赖性舒张；2）在海七鳃鳗背主动脉、海蟾蜍主动脉、短吻鳄主动脉和肺动脉、鸭主动脉和大鼠胸主动脉中产生剂量依赖性收缩；3）在鲨鱼腹主动脉、背主动脉和传入鳃动脉中产生阈值舒张；4）在海蟾蜍肺动脉、鸭肺动脉和大鼠肺动脉中产生多相收缩-舒张-收缩。用另一种激动剂对这些血管进行预收缩，除大鼠主动脉外，不影响硫氢化钠血管活性的总体模式，在大鼠主动脉中，舒张现在占主导地位。这些结果表明，H₂S是一种在系统发育上古老且多功能的血管调节分子，似乎已被机会性地利用以满足器官特异性和物种特异性的稳态需求。
