Gerriets Tibo, Stolz Erwin, Walberer Maureen, Müller Clemens, Rottger Carina, Kluge Alexander, Kaps Manfred, Fisher Marc, Bachmann Georg
Department of Radiology, Experimental Neurology Research Group, Kerckhoff-Klinik Bad Nauheim, Germany.
Stroke. 2004 Oct;35(10):2372-7. doi: 10.1161/01.STR.0000142134.37512.a7. Epub 2004 Sep 2.
Investigating focal cerebral ischemia requires animal models that are relevant to human stroke. Complications and side effects are common among these models. The present study describes potential pitfalls in 3 techniques for middle cerebral artery occlusion (MCAO) in rats using magnetic resonance imaging (MRI) and magnetic resonance angiography (MRA).
Rats were subjected to temporary MCAO for 90 minutes using the suture technique (group I; n=10) or to permanent MCAO using the suture technique (group II; n=10) or the macrosphere technique (group III; n=10). Clinical evaluation was performed after 3 hours and 24 hours. After 24 hours, animals underwent MRI and MRA to determine lesion size and the intracranial vascular status.
Hemispheric lesion volume was significantly smaller in group I (14.6%) compared with groups II (35.2%; P<0.01) and III (21.3%; P<0.05). Two animals (1 each in group II and III) did not demonstrate neurological deficits and had no lesion on MRI and a patent MCA main stem on MRA. Subarachnoid hemorrhage was detected in 2 animals (1 each in group I and II). MRA indicated a patent MCA main stem in 2 animals (group II), although both rats displayed neurological deficits. Hypothalamic infarction with subsequent pathological hyperthermia was detected in all animals in group II and in 1 rat in group III.
Model failures occurred frequently in all groups. MRI and MRA helps to identify animals that need to be excluded from experimental stroke studies.
研究局灶性脑缺血需要与人类中风相关的动物模型。这些模型中并发症和副作用很常见。本研究描述了使用磁共振成像（MRI）和磁共振血管造影（MRA）对大鼠大脑中动脉闭塞（MCAO）的3种技术中存在的潜在缺陷。
大鼠采用缝合技术进行90分钟的暂时性MCAO（I组；n = 10），或采用缝合技术（II组；n = 10）或微球技术（III组；n = 10）进行永久性MCAO。在3小时和24小时后进行临床评估。24小时后，对动物进行MRI和MRA检查，以确定病变大小和颅内血管状态。
与II组（35.2%；P < 0.01）和III组（21.3%；P < 0.05）相比，I组的半球病变体积明显较小（14.6%）。2只动物（II组和III组各1只）未表现出神经功能缺损，MRI上无病变，MRA显示大脑中动脉主干通畅。2只动物（I组和II组各1只）检测到蛛网膜下腔出血。MRA显示2只动物（II组）大脑中动脉主干通畅，尽管两只大鼠均表现出神经功能缺损。II组所有动物和III组1只大鼠检测到下丘脑梗死及随后的病理性高热。
所有组中模型失败均频繁发生。MRI和MRA有助于识别需要从实验性中风研究中排除的动物。
