Taylor R L, Verran J, Lees G C, Ward A J
Department of Biological Sciences, Department of Materials Technology Manchester M1 5GD UK.
J Mater Sci Mater Med. 1998 Jan;9(1):17-22. doi: 10.1023/a:1008874326324.
The effect of substratum roughness on the adhesion of Pseudomonas aeruginosa and Staphylococcus epidermidis was investigated using PMMA. A small increase in Ra values (0.04-1.24 microm) resulted in a significant increase (P<0.05) in bacterial attachment. Subsequent increases in surface roughness (Ra=1.86-7.89 microm) resulted in a decrease in adhesion, although adhesion was still higher than to the smooth surface. When the PMMA surfaces were coated with protein (bovine serum albumin), no difference (P<0.05) could be determined in the amount of protein adsorbed, irrespective of surface topography. However, the influence of the underlying topography on adhesion was still evident. Substratum topography is an important parameter affecting bacterial adhesion to surfaces.
使用聚甲基丙烯酸甲酯（PMMA）研究了基底粗糙度对铜绿假单胞菌和表皮葡萄球菌黏附的影响。粗糙度平均高度（Ra）值小幅增加（0.04 - 1.24微米）会导致细菌附着显著增加（P<0.05）。随后表面粗糙度增加（Ra = 1.86 - 7.89微米）会导致黏附减少，尽管黏附仍高于光滑表面。当PMMA表面涂覆蛋白质（牛血清白蛋白）时，无论表面形貌如何，吸附的蛋白质量均无差异（P<0.05）。然而，底层形貌对黏附的影响仍然明显。基底形貌是影响细菌在表面黏附的一个重要参数。
