Lantz Andrew W, Rozhkov Roman V, Larock Richard C, Armstrong Daniel W
Department of Chemistry, Iowa State University, Ames, IA 50011-3111, USA.
Electrophoresis. 2004 Aug;25(16):2727-34. doi: 10.1002/elps.200305901.
The enantiomeric separations of several very hydrophobic dihydrofuroflavones were performed and optimized using cyclodextrin-modified micellar capillary electrophoresis. Overall, the greatest enantiomeric peak-to-peak separations for the greatest number of flavones were obtained with hydroxypropyl-gamma-cyclodextrin. The effects of cyclodextrin and sodium dodecyl sulfate concentration and pH were examined in order to optimize the separation conditions. The ratio of surfactant-to-cyclodextrin concentration affected the chiral discrimination of the system significantly, with increases in the derivatized cyclodextrin concentration generally enhancing resolution. Higher efficiencies were obtained with lower concentrations of surfactant and cyclodextrin, although enantioseparation optimization often required higher concentrations to be used. A highly acidic pH was necessary to effectively suppress the electroosmotic flow when operating in the reversed polarity mode. Experiments utilizing the normal polarity mode and higher pH produced no separations.
使用环糊精修饰的胶束毛细管电泳对几种疏水性很强的二氢呋喃黄酮进行了对映体分离并优化。总体而言，使用羟丙基 - γ - 环糊精可获得大多数黄酮最大的对映体峰间分离度。研究了环糊精、十二烷基硫酸钠浓度和pH值的影响，以优化分离条件。表面活性剂与环糊精浓度之比对系统的手性识别有显著影响，衍生化环糊精浓度的增加通常会提高分离度。较低浓度的表面活性剂和环糊精可获得更高的效率，尽管对映体分离优化通常需要使用更高的浓度。在反相模式下操作时，需要高酸性pH才能有效抑制电渗流。使用正相模式和更高pH值的实验未产生分离效果。
