Jiang Chunxia, Tong Man-Yung, Armstrong Daniel W, Perera Sirantha, Bao Ye, Macdonnell Frederick M
Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, Texas, USA.
Chirality. 2009 Jan;21(1):208-17. doi: 10.1002/chir.20641.
Capillary zone electrophoresis (CZE) and micellar capillary electrophoresis (MCE) were applied for the enantiomeric separation of nine mononuclear tris(diimine)ruthenium(II) complexes as well as the separation of all stereoisomers of a dinuclear tris(diimine)ruthenium(II) complex. Nine cyclodextrin (CD) based chiral selectors were examined as run buffer additives to evaluate their effectiveness in the enantiomeric separation of tris(diimine)ruthenium(II) complexes. Seven showed enantioselectivity. Sulfated gamma-cyclodextrin (SGC), with four baseline and three partial separations, was found to be the most useful chiral selector. In CZE mode, the derivatized gamma-CDs were more effective than beta-CDs while sulfated CDs work better than carboxymethyl CDs. In MCE mode, hydroxypropyl beta-CD separated the greatest number of tris(diimine) ruthenium(II) complexes. The effects of chiral selector concentration, run buffer pH and concentration, the concentration ratio between chiral selector and other factors were investigated.
采用毛细管区带电泳（CZE）和胶束毛细管电泳（MCE）对9种单核三（二亚胺）钌（II）配合物进行对映体分离，并对一种双核三（二亚胺）钌（II）配合物的所有立体异构体进行分离。研究了9种基于环糊精（CD）的手性选择剂作为运行缓冲添加剂，以评估它们在三（二亚胺）钌（II）配合物对映体分离中的有效性。其中7种表现出对映选择性。硫酸化γ-环糊精（SGC）有4次基线分离和3次部分分离，被发现是最有用的手性选择剂。在CZE模式下，衍生化的γ-环糊精比β-环糊精更有效，而硫酸化环糊精比羧甲基环糊精效果更好。在MCE模式下，羟丙基β-环糊精分离出最多的三（二亚胺）钌（II）配合物。研究了手性选择剂浓度、运行缓冲液pH值和浓度、手性选择剂与其他因素之间的浓度比等因素的影响。
