Wang Ya-Wu, Wu Qiong, Chen Jinchun, Chen Guo-Qiang
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Biomaterials. 2005 Mar;26(8):899-904. doi: 10.1016/j.biomaterials.2004.03.035.
Hydroxyapatite (HAP) was blended into poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) to make films and scaffolds. After HAP blending, mechanical properties of PHB including compressive elastic modulus and maximum stress showed improvement and osteoblast responses including cell growth and alkaline phosphatase activity were also strengthened. On the other hand, scaffolds made of PHBHHx blended with HAP had an adverse effect. No remarkable change on degradation of PHB or PHBHHx blended with HAP, respectively, was observed in simulated body fluid. Scanning electron microscopy examination revealed that osteoblast responses to HAP incorporation may be related to surface morphology and to the exposed HAP particles on polymer surface. All these results indicated that the blending of HAP particles into PHBHHx scaffolds fabricated by salt leaching was unable to either strengthen its mechanical properties or enhance osteoblast responses. Although HAP is bioactive and osteoconductive, its blending with PHBHHx did not generate a better performance on bone reconstruction.
将羟基磷灰石（HAP）与聚（3-羟基丁酸酯）（PHB）和聚（3-羟基丁酸酯-co-3-羟基己酸酯）（PHBHHx）共混以制备薄膜和支架。HAP共混后，PHB的力学性能（包括压缩弹性模量和最大应力）有所改善，成骨细胞反应（包括细胞生长和碱性磷酸酶活性）也得到增强。另一方面，由PHBHHx与HAP共混制成的支架产生了不利影响。在模拟体液中，分别未观察到与HAP共混的PHB或PHBHHx的降解有显著变化。扫描电子显微镜检查表明，成骨细胞对HAP掺入的反应可能与表面形态以及聚合物表面暴露的HAP颗粒有关。所有这些结果表明，将HAP颗粒掺入通过盐析法制备的PHBHHx支架中既不能增强其力学性能，也不能增强成骨细胞反应。尽管HAP具有生物活性和骨传导性，但其与PHBHHx共混并未在骨重建方面产生更好的性能。
