Nablo Brian J, Schoenfisch Mark H
Department of Chemistry, University of North Carolina at Chapel Hill, 27599, USA.
Biomacromolecules. 2004 Sep-Oct;5(5):2034-41. doi: 10.1021/bm049727w.
Nitric oxide (NO) releasing sol-gel materials coated with poly(vinyl chloride) (PVC) films exhibit increased stability at ambient and physiological temperatures. The polymer overcoat, however, reduces the NO fluxes by 5-35% over the initial week of release. The variation in NO fluxes between unmodified and PVC-coated sol-gels is negligible after 7 days. The PVC polymeric layer provides controlled surface chemistry for systematic studies of the effects of NO release on bacterial adhesion. As an example, the adhesion of Pseudomonas aeruginosa and Proteus mirabilis at PVC-coated NO-releasing sol-gels is investigated. A direct NO dependence on the reduction of P. aeruginosa adhesion is observed for NO fluxes up to 20 pmol cm(-2) s(-1). Although decreased by 50% in the presence of NO release, P. mirabilis adhesion does not appear to correlate to the flux of NO release. PVC-coated NO-releasing sol-gels may prove useful for studying the effects of localized NO release on other biological and chemical systems.
涂有聚氯乙烯（PVC）薄膜的一氧化氮（NO）释放溶胶 - 凝胶材料在环境温度和生理温度下表现出更高的稳定性。然而，在释放的最初一周内，聚合物外涂层会使NO通量降低5 - 35%。7天后，未改性的和涂有PVC的溶胶 - 凝胶之间的NO通量变化可忽略不计。PVC聚合物层为系统研究NO释放对细菌粘附的影响提供了可控的表面化学性质。例如，研究了铜绿假单胞菌和奇异变形杆菌在涂有PVC的NO释放溶胶 - 凝胶上的粘附情况。对于高达20 pmol cm(-2) s(-1)的NO通量，观察到NO直接依赖于铜绿假单胞菌粘附的减少。尽管在有NO释放的情况下奇异变形杆菌的粘附减少了50%，但其粘附似乎与NO释放通量无关。涂有PVC的NO释放溶胶 - 凝胶可能被证明对研究局部NO释放对其他生物和化学系统的影响有用。
