Nichols Scott P, Schoenfisch Mark H
Department of Chemistry, Caudill Laboratories, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599.
Biomater Sci. 2013 Nov 1;1(11). doi: 10.1039/C3BM60130G.
Nitric oxide (NO) is an endogenous antibacterial agent produced by immune cells in response to pathogens. Herein, the NO fluxes necessary to reduce bacterial adhesion of different bacteria (, methicillin-resistant , , , , and ) were investigated to ascertain the sensitivity of these bacteria to NO. -nitrosothiol NO donor-modified xerogels were selected as a model NO-release surface due to their extended NO-release kinetics relative to other NO donor systems. The xerogels were coated with poly(vinyl chloride) (PVC) to achieve consistent surface energy between NO-releasing and control substrates. Fibrinogen was pre-adsorbed to these materials to more accurately mimic conditions encountered in blood and promote bacteria adhesion. Nitric oxide fluxes ranging from 20-50 pmol cm s universally inhibited the bacterial adhesion by >80% for each strain studied. Maximum bacteria killing activity (reduced viability by 85-98%) was observed at the greatest NO payload (1700 nmol cm).
一氧化氮（NO）是免疫细胞针对病原体产生的一种内源性抗菌剂。在此，研究了减少不同细菌（耐甲氧西林金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、粪肠球菌和金黄色葡萄球菌）细菌黏附所需的NO通量，以确定这些细菌对NO的敏感性。由于相对于其他NO供体系统，其具有延长的NO释放动力学，因此选择N-亚硝基硫醇NO供体修饰的干凝胶作为NO释放表面模型。用聚氯乙烯（PVC）涂覆干凝胶，以使NO释放底物和对照底物之间具有一致的表面能。将纤维蛋白原预先吸附到这些材料上，以更准确地模拟血液中遇到的情况并促进细菌黏附。对于所研究的每个菌株，20 - 50 pmol cm² s⁻¹ 的NO通量普遍抑制细菌黏附>80%。在最大NO负载量（1700 nmol cm²）下观察到最大的细菌杀伤活性（活力降低85 - 98%）。
