嗜碱芽孢杆菌肽聚糖诱导干扰素-α介导的牛痘病毒复制抑制。

Bacillus alcalophilus peptidoglycan induces IFN-alpha-mediated inhibition of vaccinia virus replication.

作者信息

Liu Ge, Zhai Qingzhu, Schaffner Dustin, Popova Taissia, Hayford Alice, Bailey Charlie, Alibek Ken

机构信息

Advanced Biosystems, Inc., George Mason University, 10900 University Blvd., MSN 1A8 Manassas, VA 20110, USA.

出版信息

FEMS Immunol Med Microbiol. 2004 Oct 1;42(2):197-204. doi: 10.1016/j.femsim.2004.05.002.

DOI:10.1016/j.femsim.2004.05.002

PMID:15364104

Abstract

Bacterial products such as cell walls (CW) and peptidoglycan (PGN) are known to activate macrophages and NK cells during microbial infections. In this report, we demonstrated that whole CW and PGN of four Gram-positive bacteria are capable of enhancing the anti-poxviral activity of murine macrophage RAW 264.7 cells. Among the major Bacillus alcalophilus CW components, PGN contributes the most to antiviral activity and induces remarkably higher levels of IFN-alpha. Anti-IFN-alpha/beta antibody, but not anti-IFN-gamma, anti-IFN-gamma receptor, or anti-IL-12, reversed the PGN-induced inhibition of vaccinia virus replication and reduced nitric oxide (NO) production. Our data thus suggest that PGN induce antiviral activity through IFN-alpha and to a lesser extent, through NO production.

摘要

已知细菌产物如细胞壁（CW）和肽聚糖（PGN）在微生物感染期间可激活巨噬细胞和自然杀伤细胞。在本报告中，我们证明了四种革兰氏阳性菌的完整CW和PGN能够增强小鼠巨噬细胞RAW 264.7细胞的抗痘病毒活性。在嗜碱芽孢杆菌主要的CW成分中，PGN对抗病毒活性的贡献最大，并诱导产生显著更高水平的干扰素-α。抗干扰素-α/β抗体，而非抗干扰素-γ、抗干扰素-γ受体或抗白细胞介素-12，可逆转PGN诱导的痘苗病毒复制抑制并减少一氧化氮（NO）的产生。因此，我们的数据表明PGN通过干扰素-α诱导抗病毒活性，且在较小程度上通过NO的产生发挥作用。