Rao J Venkateswara, Kavitha P
Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Hyderabad 500-007, India.
Environ Res. 2004 Nov;96(3):323-7. doi: 10.1016/j.envres.2004.02.014.
The acute toxicity of azodrin (monocrotophos, an organophosphorus insecticide) was determined on a soil organism, Eisenia foetida. The median lethal concentrations (LC50) were derived from a 48-h paper contact test and from artificial soil tests. The LC50 of azodrin in the paper contact test was 0.46 +/- 0.1 microg cm(-2) (23 +/- 6 mg L(-1)) and those in the 7- and 14-day artificial soil tests were 171 +/- 21 and 132 +/- 20 mg kg(-1), respectively. The neurotoxic potentiality of azodrin was assessed by using a marker enzyme, acetylcholinesterase (AChE; EC 3.1.1.7) in both in vitro and in vivo experiments. The progressive signs of morphological destruction are correlated with percentage inhibition of AChE in the in vivo experiments. The kinetics of AChE activity in the presence and absence of azodrin indicated that the toxicant is competitive in nature. This study demonstrated that azodrin causes concentration-dependent changes in the morphology and AChE activity of the earthworm E. foetida.
测定了久效磷（一种有机磷杀虫剂）对土壤生物赤子爱胜蚓的急性毒性。通过48小时滤纸接触试验和人工土壤试验得出半数致死浓度（LC50）。久效磷在滤纸接触试验中的LC50为0.46±0.1微克/平方厘米（23±6毫克/升），在7天和14天人工土壤试验中的LC50分别为171±21毫克/千克和132±20毫克/千克。在体外和体内实验中，通过使用标记酶乙酰胆碱酯酶（AChE；EC 3.1.1.7）评估久效磷的神经毒性潜力。在体内实验中，形态破坏的渐进迹象与AChE的抑制百分比相关。在有和没有久效磷存在的情况下AChE活性的动力学表明，该毒物本质上具有竞争性。本研究表明，久效磷会导致赤子爱胜蚓的形态和AChE活性发生浓度依赖性变化。
