Key Lab of Aquatic Animal Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Fish Physiol Biochem. 2012 Jun;38(3):769-75. doi: 10.1007/s10695-011-9559-z. Epub 2011 Oct 11.
To investigate the effect of trichlorfon on oxidative stress and hepatocyte apoptosis of Carassius auratus gibelio in vivo, the fish were exposed to 0, 0.5, 1.0, 2.0 and 4.0 mg l(-1) trichlorfon concentrations for 30 days. At the end of the experiment, plasm antioxidant activities, hepatic total nitric oxide synthase (T-NOS) activity, xanthine oxidase (XOD) activity and hepatocyte apoptosis rate were analyzed. The results showed hepatic T-NOS activities, XOD activities and hepatocyte apoptosis rate were increased with trichlorfon concentration increase. The hepatic MDA content was increased significantly at 4.0 mg l(-1) trichlorfon treatment. The disturbance of antioxidative balance was observed based on the monitoring of the plasm superoxide dismutase (SOD), catalase (CAT) activities and vitamin E levels. The current study revealed that trichlorfon influenced fish plasma antioxidative status, caused lipid peroxidation (LPO) then resulted in hepatocytes apoptosis.
为了研究三氯氧磷对体内吉富罗非鱼的氧化应激和肝细胞凋亡的影响，将鱼暴露于 0、0.5、1.0、2.0 和 4.0mg/L 的三氯氧磷浓度下 30 天。实验结束时，分析了血浆抗氧化活性、肝总一氧化氮合酶（T-NOS）活性、黄嘌呤氧化酶（XOD）活性和肝细胞凋亡率。结果表明，随着三氯氧磷浓度的增加，肝 T-NOS 活性、XOD 活性和肝细胞凋亡率增加。4.0mg/L 三氯氧磷处理时，肝 MDA 含量显著增加。通过监测血浆超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性和维生素 E 水平，观察到抗氧化平衡的紊乱。本研究表明，三氯氧磷影响鱼类血浆抗氧化状态，引起脂质过氧化（LPO），进而导致肝细胞凋亡。
