花生中的α-半乳糖苷酶和β-半乳糖苷酶活性以及寡糖含量

Alpha and beta-galactosidase activities and oligosaccharide content in peanuts.

作者信息

Bryant R J, Rao D R, Ogutu S

机构信息

Department of Food and Animal Science, Alabama A&M University, 4900 Meridian St. N, Normal, AL 35762, USA.

出版信息

Plant Foods Hum Nutr. 2003;58(3):213-23. doi: 10.1023/b:qual.0000040351.01307.ed.

DOI:10.1023/b:qual.0000040351.01307.ed

PMID:15366262

Abstract

Thirty-three peanut cultivars were examined for their alpha-1,6 and beta-1,4 galactosidase activities and oligosaccharide contents along with proximate compositions. The average moisture, protein, fat, ash, and carbohydrate contents were: 4.9%, 26.6%, 43.1%, 2.3% and 23.1%, respectively. The corresponding coefficients of variation were: 5.2%, 10.1%, 7.2%, 7.8% and 15.7%, respectively. Raffinose and stachyose contents (%) ranged from 0.05 to 0.12 and 0.31 to 0.61, respectively. The specific activity (micromol product/min/mg protein) of crude preparation of alpha-galactosidase for the 33 cultivars ranged from 1.096 to 2.784 for the non-germinated seeds and from not being detected in some samples up to 2.432 for the germinated seeds; the mean values for non-germinated and germinated seeds were: 1.781 and 1.410, respectively. The specific activity of beta-galactosidase ranged from 0.101 to 1.727 in the non-germinated seeds and from not being detected in some samples up to 0.898 in the germinated seeds. Germination decreased the activity of both galactosidases significantly (p < or = 0.05).

摘要

对33个花生品种进行了α-1,6和β-1,4半乳糖苷酶活性、低聚糖含量以及近似成分的检测。平均水分、蛋白质、脂肪、灰分和碳水化合物含量分别为：4.9%、26.6%、43.1%、2.3%和23.1%。相应的变异系数分别为：5.2%、10.1%、7.2%、7.8%和15.7%。棉子糖和水苏糖含量（%）分别在0.05至0.12以及0.31至0.61之间。33个品种的α-半乳糖苷酶粗制品的比活性（微摩尔产物/分钟/毫克蛋白质），未发芽种子的范围为1.096至2.784，发芽种子的范围在某些样品中未检测到至2.432；未发芽种子和发芽种子的平均值分别为1.781和1.410。β-半乳糖苷酶的比活性在未发芽种子中为0.101至1.727，在发芽种子中在某些样品中未检测到至0.898。发芽显著降低了两种半乳糖苷酶的活性（p≤0.05）。