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脱脂花生饼中多糖的提取、纯化及初步表征

Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes.

作者信息

Liu Hongzhi, Jiang Nan, Liu Li, Sheng Xiaojing, Shi Aimin, Hu Hui, Yang Ying, Wang Qiang

机构信息

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, China.

Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Risk Assessment Lab for Agro-Products (Beijing)/Ministry of Agriculture, Beijing 100097, China.

出版信息

Molecules. 2016 Jun 1;21(6):716. doi: 10.3390/molecules21060716.

DOI:10.3390/molecules21060716
PMID:27258246
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6274344/
Abstract

The hot-water extraction, purification and characterization of polysaccharides from defatted peanut cake (PPC) were investigated in this study. A Box-Behnken factorial design (BBD) was used to investigate the effects of three independent variables, namely extraction temperature (X₁), extraction time (X₂) and ratio of water to raw material (X₃). The optimum conditions were 85 °C, 3 h and 20:1 (mL/g) respectively. Regression analysis was done to reveal the experimental results which include 34.97% extraction rate while the value verified under these conditions was 34.49%. The crude PPC was sequentially further purified by Sephadex G-100 chromatography, and one purified fraction was obtained. The PPC purified fraction was characterized by FT-IR, HPAEC; SEC-MALLS. The average molecular weight of the PPC purified fraction was 2.383 × 10⁵ Da. The polysaccharide was mainly composed of glucose, galactose, arabinose and xylose. The PPC have the typical absorption of polysaccharide.

摘要

本研究对脱脂花生饼（PPC）中多糖的热水提取、纯化及表征进行了研究。采用Box-Behnken析因设计（BBD）考察了3个自变量，即提取温度（X₁）、提取时间（X₂）和料液比（X₃）的影响。最佳条件分别为85℃、3小时和20:1（mL/g）。进行回归分析以揭示实验结果，其中提取率为34.97%，在这些条件下验证的值为34.49%。粗制PPC依次通过Sephadex G-100柱色谱进一步纯化，得到一个纯化级分。通过傅里叶变换红外光谱（FT-IR）、高效阴离子交换色谱（HPAEC）和体积排阻色谱-多角度激光光散射联用（SEC-MALLS）对PPC纯化级分进行了表征。PPC纯化级分的平均分子量为2.383×10⁵ Da。该多糖主要由葡萄糖、半乳糖、阿拉伯糖和木糖组成。PPC具有多糖的典型吸收峰。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/da39b8f8f270/molecules-21-00716-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/f1a803906ea1/molecules-21-00716-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/4df6d204ce79/molecules-21-00716-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/ea92386d550e/molecules-21-00716-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/f62a45272174/molecules-21-00716-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/7a5bad169a6b/molecules-21-00716-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/4e9af0b4f014/molecules-21-00716-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/da39b8f8f270/molecules-21-00716-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/f1a803906ea1/molecules-21-00716-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/4df6d204ce79/molecules-21-00716-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/ea92386d550e/molecules-21-00716-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/f62a45272174/molecules-21-00716-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/7a5bad169a6b/molecules-21-00716-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/4e9af0b4f014/molecules-21-00716-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/6274344/da39b8f8f270/molecules-21-00716-g007.jpg

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