Wilson J M, Zalesky D D, Looney C R, Bondioli K R, Magness R R
Department of Animal Science, Texas A&M University, College Station 77843.
Biol Reprod. 1992 Feb;46(2):295-300. doi: 10.1095/biolreprod46.2.295.
Bovine embryos recovered from superovulated donors on Days 8-18 postestrus were cultured in vitro in a tissue perifusion system to quantify hormone secretion. Embryos were cultured for 24 h at 37 degrees C in Ham's F-10 medium supplemented 5% v/v with heat-treated, charcoal-stripped calf serum; 100 IU/ml penicillin; and 100 micrograms/ml streptomycin. The medium was saturated with 5% CO2 in air and perifused at 50 microliters/min (3 ml/h). Estrone (E1) estradiol (E2), progesterone (P4), prostaglandin E2 (PGE2), and prostacyclin (PGI2) were quantified by RIA in 6-h pools of perifusate fractions. Estrone was measurable (pg/h/embryo; mean +/- SE) on Days 13 (10.80 +/- 4.56) and 15 (34.80 +/- 9.80); E2 on Days 11 (36.80), 12 (81.28 +/- 29.80), 13 (11.75 +/- 4.09), 15 (157.20 +/- 112.60), and 16 (30.26 +/- 8.76); and P4 (ng/h/embryo) on Days 13 (0.5-1.0) and 17 (approximately 1.5). PGE2 was secreted by Day 10 bovine embryos during the last 6 h of culture (19-24 h) and throughout culture for Day 11-18 embryos. The rate of PGE2 secretion increased (p less than 0.05) over the previous days(s) at Days 13 and 17. The mean (+/- SE) secretion rates (pg/h/embryo) for the 24-h culture by embryonic ages were as follows: Day 11 (63.39 +/- 14.61), 12 (172.10 +/- 30.90), 13 (3094.08 +/- 283.35), 14 (1633.89 +/- 49.98), 15 (3739.23 +/- 1082.79), 16 (4955.37 +/- 1381.83), 17 (11893.23 +/- 1188.48), and 18 (13827.99 +/- 3587.88).(ABSTRACT TRUNCATED AT 250 WORDS)
从发情后第8 - 18天超排供体牛回收的胚胎，在体外组织灌流系统中培养以定量激素分泌。胚胎在含5%（体积/体积）经热处理、活性炭处理的小牛血清、100 IU/ml青霉素和100μg/ml链霉素的Ham's F - 10培养基中于37℃培养24小时。培养基用5%二氧化碳饱和空气，并以50微升/分钟（3毫升/小时）的速度灌流。通过放射免疫分析法对灌流液馏分6小时收集池中的雌酮（E1）、雌二醇（E2）、孕酮（P4）、前列腺素E2（PGE2）和前列环素（PGI2）进行定量。在第13天（10.80±4.56）和第15天（34.80±9.80）可检测到雌酮（皮克/小时/胚胎）；在第11天（36.80）、12天（81.28±29.80）、13天（11.75±4.09）、15天（157.20±112.60）和16天（30.26±8.76）可检测到E2；在第13天（0.5 - 1.0）和第17天（约1.5）可检测到P4（纳克/小时/胚胎）。第10天的牛胚胎在培养的最后6小时（19 - 24小时）分泌PGE2，第11 - 18天的胚胎在整个培养过程中都分泌。在第13天和第17天，PGE2的分泌速率比前几天有所增加（p<0.05）。按胚胎日龄划分的24小时培养的平均（±SE）分泌速率（皮克/小时/胚胎）如下：第11天（63.39±14.61）、12天（172.10±30.90）、13天（3094.08±283.35）、14天（1633.89±49.98）、15天（3739.23±1082.79）、16天（4955.37±1381.83）、17天（11893.23±1188.48）和18天（13827.99±3587.88）。（摘要截断于250字）
