Vincent D L, Meredith S, Inskeep E K
Endocrinology. 1986 Aug;119(2):527-9. doi: 10.1210/endo-119-2-527.
Effects of treatment with progesterone early after estrus and the age of the blastocyst in the uterus on day-6 postestrus on uteroovarian venous concentration of prostaglandin E2 (PGE2) during days 7-12 postestrus were examined. At estrus, 24 ewes were assigned randomly to mating with fertile rams and daily injections of progesterone from days 1-3 postestrus, mating with fertile rams and daily injections of vehicle from days 1-3 postestrus, no mating, injection of progesterone from days 1-3 postestrus and transfer of a day-10 blastocyst into the uterus on day-6 postestrus, and no mating, injection of vehicle from days 1-3 postestrus and transfer of a day-10 blastocyst into the uterus on day-6 postestrus. PGE2 increased beginning on day 8 in progesterone-treated ewes that received a day-10 blastocyst on day-6 postestrus, but did not increase until day 12 in mated ewes with confirmed pregnancy. PGE2 remained at basal concentrations in mated progesterone-treated ewes (with a day-6 blastocyst on day-6) and showed only a transient rise on day-11 in vehicle-treated ewes which received a day-10 blastocyst on day-6 postestrus. It is concluded that progesterone regulates the ability of the uterus to release PGE2 in response to a blastocyst of appropriate age.
研究了发情后早期用孕酮处理以及发情后第6天子宫内囊胚的年龄对发情后第7 - 12天子宫卵巢静脉中前列腺素E2（PGE2）浓度的影响。在发情期，将24只母羊随机分为四组：发情后第1 - 3天与可育公羊交配并每日注射孕酮组；发情后第1 - 3天与可育公羊交配并每日注射赋形剂组；不交配，发情后第1 - 3天注射孕酮并在发情后第6天将第10天的囊胚移植到子宫组；不交配，发情后第1 - 3天注射赋形剂并在发情后第6天将第10天的囊胚移植到子宫组。在发情后第6天接受第10天囊胚的孕酮处理母羊中，PGE2从第8天开始升高，但在确认怀孕的交配母羊中直到第12天才升高。在交配的孕酮处理母羊（发情后第6天有第6天的囊胚）中，PGE2保持在基础浓度，并且在发情后第6天接受第10天囊胚的赋形剂处理母羊中，仅在第11天出现短暂升高。得出的结论是，孕酮调节子宫响应适当年龄囊胚释放PGE2的能力。
