Shutt D A, Lopata A
Fertil Steril. 1981 Apr;35(4):413-6. doi: 10.1016/s0015-0282(16)45435-x.
Following in vitro fertilization of human preovulatory oocytes from spontaneously ovulating women, determinations were made of the secretion into the culture medium of progesterone, estradiol, human chorionic gonadotropin (hCG), and the prostaglandins (PG) E2 and F2 alpha, over a 3- to 4-day period of embryo development. It was found that the corona cells associated with the egg could account, between days 2 and 3, for a mean daily secretion of 50 ng of progesterone and approximately 100 pg of estradiol, PGE2, and PGF2 alpha, respectively. Mechanical removal of the corona cells after about 48 hours for the examination of the egg for cleavage reduced the mean amount of progesterone produced on day 3 to 2 ng, and a concomitant decrease in estradiol. PGE2, and PGF2 alpha was observed. Steroid secretion could be restored on day 3 to more than 50% of that secreted on day 2 by returning some detached corona cells to the culture medium containing the embryo. hCG was not detected (less than 2 mIU/ml) in the culture medium at any stage of embryo culture over the 3- to 4-day period.
对自然排卵女性的人排卵前卵母细胞进行体外受精后，在胚胎发育的3至4天内，测定了孕酮、雌二醇、人绒毛膜促性腺激素（hCG）以及前列腺素（PG）E2和F2α向培养基中的分泌情况。结果发现，与卵子相关的放射冠细胞在第2天至第3天期间，平均每天分别分泌50 ng孕酮以及约100 pg雌二醇、PGE2和PGF2α。在约48小时后为检查卵子是否分裂而机械去除放射冠细胞，使第3天产生的孕酮平均量降至2 ng，同时观察到雌二醇、PGE2和PGF2α也随之减少。通过将一些分离的放射冠细胞放回含有胚胎的培养基中，第3天的类固醇分泌可恢复至第2天分泌量的50%以上。在3至4天的胚胎培养的任何阶段，培养基中均未检测到hCG（低于2 mIU/ml）。
