Fridman Eyal, Carrari Fernando, Liu Yong-Sheng, Fernie Alisdair R, Zamir Dani
Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Hebrew University of Jerusalem, Post Office Box 12, Rehovot 76100, Israel.
Science. 2004 Sep 17;305(5691):1786-9. doi: 10.1126/science.1101666.
To explore natural biodiversity we developed and examined introgression lines (ILs) containing chromosome segments of wild species (Solanum pennellii) in the background of the cultivated tomato (S. lycopersicum). We identified Brix9-2-5, which is a S. pennellii quantitative trait locus (QTL) that increases sugar yield of tomatoes and was mapped within a flower- and fruit-specific invertase (LIN5). QTL analysis representing five different tomato species delimited the functional polymorphism of Brix9-2-5 to an amino acid near the catalytic site of the invertase crystal, affecting enzyme kinetics and fruit sink strength. These results underline the power of diverse ILs for high-resolution perspectives on complex phenotypes.
为了探索自然生物多样性，我们开发并检测了在栽培番茄（S. lycopersicum）背景下含有野生种（潘那利番茄，Solanum pennellii）染色体片段的渐渗系（ILs）。我们鉴定出了Brix9-2-5，它是潘那利番茄的一个数量性状基因座（QTL），可提高番茄的糖分产量，并且被定位在一个花和果实特异性转化酶（LIN5）内。对五个不同番茄品种的QTL分析将Brix9-2-5的功能多态性限定在转化酶晶体催化位点附近的一个氨基酸上，影响酶动力学和果实库强度。这些结果强调了多样渐渗系对于复杂表型进行高分辨率研究的作用。
