与伯氏疏螺旋体外膜蛋白OspB反应的单克隆抗体的补体介导的体外杀菌活性。

Complement-mediated in vitro bactericidal activity of monoclonal antibodies reactive with outer-surface-protein OspB of Borrelia burgdorferi.

作者信息

Cevenini R, Sambri V, Massaria F, La Placa M, Brocchi E, De Simone F

机构信息

Istituto di Microbiologia, University of Bologna, Italy.

出版信息

FEMS Microbiol Lett. 1992 Jan 1;69(2):147-52. doi: 10.1016/0378-1097(92)90619-y.

DOI:10.1016/0378-1097(92)90619-y

PMID:1537543

Abstract

Murine monoclonal antibodies (mAbs) were obtained against the outer-surface-protein OspA and OspB and against the 41-kDa flagellar antigen of Borrelia burgdorferi. The specificity of mAb was determined by the Western blotting technique and the surface association of the antigens was inferred by immunofluorescence of living bacteria. In an in vitro assay in the presence of complement, two mAbs reactive with the Ospa were able to kill borreliae, whereas several mAbs reactive with the OspA as well as with the 41-kDa flagellar protein were not.

摘要

获得了针对伯氏疏螺旋体外膜蛋白OspA和OspB以及41 kDa鞭毛抗原的鼠单克隆抗体（mAb）。通过蛋白质印迹技术确定mAb的特异性，并通过活细菌的免疫荧光推断抗原的表面关联性。在补体存在的体外试验中，两种与Ospa反应的mAb能够杀死疏螺旋体，而几种与OspA以及41 kDa鞭毛蛋白反应的mAb则不能。